Which gene should I target for my study, TSC1 or TSC2?

While both genes form the functional TSC complex, mutations in TSC2 are more common and often associated with a more severe clinical phenotype. Your research goals will determine the most appropriate choice. We have engineered robust models for both targets to meet your needs.

Can these cells be used to test mTOR inhibitors like rapamycin?

Absolutely. These cell lines are an ideal system for testing the efficacy and dose-response of mTOR inhibitors and their analogs.

Do you support large-scale or custom projects?

Yes, we support research at any scale. We can supply our catalog models in bulk quantities for high-throughput screening campaigns. Additionally, our scientific services team specializes in end-to-end custom model generation. Please contact us to discuss the specific requirements of your project.

Can I request a gene knockout in a cell line of my choice?

Yes, creating custom knockouts is a core part of our service offerings. Our expert team can target any gene of interest—including, but not limited to, TSC1 and TSC2—in virtually any cell line you require for your research.

Can you create a model with a specific patient-derived mutation not in your catalog?

Yes, this is our area of expertise. Our custom engineering services are designed to create models with precise, clinically relevant mutations. We are equipped to engineer specific point mutations, frameshifts, insertions, or deletions into your chosen cell line. We encourage you to contact our scientific team to initiate a confidential discussion about your target mutation, and we will develop a customized project proposal for you.

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Tuberous Sclerosis Complex Cell Model Products

Introduction Types Advantages Applications FAQs Related Product Sections Product List

Introduction

Tuberous Sclerosis Complex (TSC) is a multifaceted genetic disorder driven by inactivating mutations in the TSC1 or TSC2 genes, leading to hyperactivation of the critical mTOR signaling pathway. This dysregulation results in the growth of benign tumors across multiple organ systems, including the brain, kidneys, heart, and skin, and is strongly associated with epilepsy, cognitive impairment, and autism spectrum disorder.

Advancing our understanding and developing effective therapeutics for TSC requires precise, reliable, and clinically relevant cellular models. Creative Biolabs provides researchers with a comprehensive suite of expertly engineered and rigorously validated TSC cell models. Our platforms empower you to investigate fundamental disease mechanisms, screen novel therapeutic compounds, and push the boundaries of TSC research with confidence. Contact our scientific team to discover how our portfolio of TSC cell models can advance your research and address your specific project needs.

To view our entire product catalog, please visit the 'Product List' section.

Types

Our product portfolio is thoughtfully curated to provide researchers with the precise tools required for their TSC studies.

Types	Description
TSC1 & TSC2 Knockout Cell Lines	These CRISPR-engineered cell lines (e.g., HEK293T, U-2 OS, HAP1) harbor complete loss-of-function mutations in TSC1 or TSC2. Each model is delivered with its corresponding parental wild-type line to enable experimentally clean and definitive results.
Isogenic Control iPSCs	The essential counterpart to our patient-derived models. Using precision gene editing, we correct the specific disease-causing mutation in the patient's iPSCs, generating a genetically identical wild-type control for the most rigorous comparative analysis possible.
Differentiated Neuronal & Glial Cells	As a specialized service, we provide ready-to-use neural lineages derived from our TSC iPSC lines. This includes expertly differentiated neuronal progenitors, mature neurons, and astrocytes, saving your lab valuable time and resources.
Custom Model Generation	Our team offers fully customized model development to meet your unique specifications. We perform bespoke gene editing—from knockouts and knock-ins to specific patient mutation replications—in your cell line of choice to create a model tailored precisely to your research.

Advantages

Partnering with Creative Biolabs provides a distinct advantage, granting your research program direct access to our team of seasoned scientific experts and a suite of state-of-the-art technology platforms designed to accelerate discovery

Unambiguous Results

Isogenic controls eliminate confounding effects from genetic background, providing the clearest possible data for interpreting gene function and drug response.

Accelerated Timelines

Accelerate your research timeline by skipping the demanding process of in-house model development. With our ready-to-use, validated models, your team can instantly concentrate on answering key scientific questions.

Clinical Relevance

Our models accurately recapitulate the core molecular pathology of TSC—mTORC1 hyperactivation—making them ideal platforms for preclinical drug screening and biomarker discovery.

Reproducibility & Reliability

Strict manufacturing and QC processes ensure high lot-to-lot consistency, a critical factor for long-term studies and multi-site collaborations.

Expert Support

Gain direct access to our team of cell model specialists for technical support, experimental design consultation, and guidance on selecting the optimal model for your study.

Broad Applications

Creative Biolabs' TSC cell models are versatile tools suitable for a wide range of research applications:

Applications	Description
Fundamental Disease Mechanistic Studies	Investigate the downstream consequences of TSC1/TSC2 loss on cell growth, proliferation, autophagy, and metabolism.
High-Throughput Drug Screening	Screen libraries of small molecules, biologics, or novel mTOR inhibitors to identify promising therapeutic candidates.
Target Identification & Validation	Uncover and validate new downstream targets within the mTOR pathway that could be leveraged for therapeutic intervention.
Biomarker Discovery	Identify and test potential pharmacodynamic or disease-state biomarkers in a controlled cellular environment.
Neurobiology Research	Utilize iPSC-derived neurons and astrocytes to model TSC-associated neurological phenotypes like neuronal hyperexcitability, migration defects, and astrogliosis.
Organoid Development	Serve as a foundational source for generating 3D organoid models that better mimic in vivo tissue architecture and tumor microenvironments.

A picture that presents the Signaling pathways associated with mTOR activity. (Bovari-Biri, et al., 2023) (OA Literature)

Fig.1 Signaling pathways associated with mTOR activity. ¹

FAQs

Which gene should I target for my study, TSC1 or TSC2?
While both genes form the functional TSC complex, mutations in TSC2 are more common and often associated with a more severe clinical phenotype. Your research goals will determine the most appropriate choice. We have engineered robust models for both targets to meet your needs.
Can these cells be used to test mTOR inhibitors like rapamycin?
Absolutely. These cell lines are an ideal system for testing the efficacy and dose-response of mTOR inhibitors and their analogs.
Do you support large-scale or custom projects?
Yes, we support research at any scale. We can supply our catalog models in bulk quantities for high-throughput screening campaigns. Additionally, our scientific services team specializes in end-to-end custom model generation. Please contact us to discuss the specific requirements of your project.
Can I request a gene knockout in a cell line of my choice?
Yes, creating custom knockouts is a core part of our service offerings. Our expert team can target any gene of interest—including, but not limited to, TSC1 and TSC2—in virtually any cell line you require for your research.
Can you create a model with a specific patient-derived mutation not in your catalog?
Yes, this is our area of expertise. Our custom engineering services are designed to create models with precise, clinically relevant mutations. We are equipped to engineer specific point mutations, frameshifts, insertions, or deletions into your chosen cell line. We encourage you to contact our scientific team to initiate a confidential discussion about your target mutation, and we will develop a customized project proposal for you.

The path to new therapies for Tuberous Sclerosis Complex is built on a foundation of robust, reliable, and biologically relevant research. Let Creative Biolabs provide that foundation for you. Empower your team with the highest quality tools and accelerate your journey from hypothesis to impactful discovery. Contact us today to speak with a scientist about how our advanced cell models can drive your project to its next milestone.