Basic Neuroscience Assay Services

Overview Service Features Applications FAQs Scientific Resources Related Services

In the past decades, a series of medical and technical advances have allowed researchers not only to localize areas involved in specific functions but also to describe the mechanisms underlying these functions. In this context, the development of technologies will bring neuroscience a golden age. Various assays have become unignorable in neuroscience.

Overview of Basic Neuroscience Assay

Neuroscience assays are essential tools in research for understanding the structure and function of the nervous system. These assays typically involve the use of various techniques and technologies to measure the activity and function of neurons, glial cells, and other components of the nervous system. These assays can broadly be categorized based on the level of analysis they target, such as molecular, cellular, systems, behavioral, and computational approaches.

Some common neuroscience assays include:

Electrophysiology assays, which involve the measurement of electrical activity in the brain and nervous system.
Imaging assays, such as MRI and fMRI, which allow researchers to visualize brain structure and activity in real time.
Biochemical assays, which involve the measurement of various molecules and substances in the brain and nervous system.

Overall, neuroscience assays play a crucial role in advancing our understanding of the brain and nervous system and developing new treatments for neurological disorders. By combining different assays and approaches, researchers can gain a comprehensive understanding of the complex functions of the brain and how they are affected by various factors.

Service Features

Below is an overview of some of the neuroscience assays we offer.

Calcium Assay Service

Calcium ions (Ca²⁺) serve as a ubiquitous second messenger within cells across all domains of life, modulating a wide array of cellular processes. Hundreds of proteins have evolved to modulate intracellular calcium levels and to transduce changes in calcium concentrations into downstream cellular signals. Monitoring intracellular calcium levels is a valuable technique for modern drug discovery, allowing functional measurements of target proteins and calcium-mediated physiological pathways.

Fig.1 Ca²⁺- and light-gated transcriptional reporters of activated neurons.¹

Neurotoxicity Screening Service

The developing brain is uniquely sensitive to environmental insults as it is critically dependent on precisely orchestrated developmental processes that must occur in a particular sequence at the right time and location. The prevalence of diagnosed neurodevelopmental disorders is increasing worldwide and fuels public concern regarding the lack of developmental neurotoxicity (DNT) data for many chemicals. Among the thousands of known chemicals, there is only a small fraction proven to cause DNT in humans. The paucity of DNT data is partial since DNT evaluation is not a mandatory requirement unless triggered by evidence of developmental toxicity involving the nervous system, or neurotoxicity, or endocrine disruption in systemic toxicity studies in adult rodents. Neurotoxicity screening is thus unignored in neuro-drug development.

Fig.2 Example plate layout for screening chemicals.²

High Content Screening Service

High content screening (HCS) has gained tremendous popularity in the past few years in the drug discovery industry from advances in fluorescence microscopy and automated screening technologies. The unique architecture of post-mitotic neurons with their elaborate dendritic trees and far-reaching axons imposes extraordinary demands on the mitochondrial system for satisfying the neuron's need for energy, calcium buffering, neurotransmitter metabolism, and other physiological processes. HCS provides an opportunity to rapidly screen chemical or siRNA libraries by imaging subcellular and molecular events of individual cells with an automated fluorescent microscope. HCS is an assay platform capable of multiplexed, high-throughput, and phenotypic screening of small molecules for modulatory effects on neuronal metastasis.

Immunostaining Services

The direct method of immunostaining allowed a single antibody, which is conjugated to an enzyme, to interact with an antigen present on the cell of interest. A substrate is then added, and after a reaction mediated by the conjugated enzyme, the substrate will fluoresce or form an insoluble color product deposited near the antibody. The indirect, or amplifying, technique has proven to be more efficient than the direct technique, particularly in the setting of frozen section tissue examination.

Multi Electrode Arrays (MEA) Measurements of Neurons

Substrate-integrated MEAs allow simultaneous extracellular recording of electrical activity from many individual sites in electrically active tissues. MEA systems offer a great deal of flexibility regarding biological tissue and experimental design. A wide variety of electrically excitable biological tissues may be placed onto the MEA. This includes primary cultures of nervous system tissue from many different regions, i.e. mouse frontal cortex cells, tissue slices (e.g. hippocampal slice), or retinas.

Ion Channel Screening Services

Ion channels are a very important target class because of their involvement in many physiological processes. Historically, ion channel targeted drug discovery has been hampered by the unavailability of high-throughput platforms utilizing electrophysiological techniques for the characterization of compound activity. Two major approaches have formed the basis for cell-based ion channel screening: the highest throughput optical assays representing indirect measures of ion channel function, and the introduction of higher-throughput electrophysiological methodologies. In combination with non-electrophysiological but higher throughput methods, the implementation of these technologies provides a highly integrated approach for ion channel targeted drug discovery and will likely have a significant impact on the discovery of new ion channel targeted therapeutics.

Sparse-labeling Services

A central feature of the nervous system is the presence of an enormous diversity of neurons with different morphologies, molecular profiles, and physiological properties. Neurons are densely packed in the brain, and their intermingled dendritic and axonal processes preclude the visualization of their morphologies. Thus, it often requires a sparse labeling method that randomly labels a small subset of neurons to highlighting their dendritic and axonal processes.

Neuronal Activity Monitor Services

A central challenge in systems neuroscience is linking animal behavior to underlying neuronal circuitry. Only a small percentage of neurons are activated during a specific task or sensory experience, and these can be embedded within a large population of nonactivated neurons that have similar morphology and genetic features. Once these neurons are identified, their functional and molecular properties can provide insight into how the brain performs computation, promotes or inhibits actions, or encodes sensory stimuli. Monitoring neuronal activity will give a chance to reveal the mechanism underlying these challenges.

High-throughput Phenotypic Screen Services

Phenotypic omics is broadly defined as the acquisition of organism-wide high-dimensional phenotypic data, which will be a natural evolution and supplementation of existing molecular omics paradigms in drug discovery. Phenomic-level data can help to understand genomic variants underlying phenotypes, pleiotropy of responses to pharmaceuticals as well as provide new high-throughput and content-rich screening paradigms in drug discovery.

Fig.3 Phenotypic drug discovery compound screening and validation.³

Focusing on neuroscience over years, Creative Biolabs has established a great industry reputation. Our technology platform has integrated a variety of basic neuroscience assays. With rich experience and excellent expert teams, we are capable of providing mature neuroscience assay services to global customers. If you are interested in any one of the neuroscience assay services on our website or focusing on other content of neuroscience, please don't hesitate to contact us for more information.

Applications of Basic Neuroscience Assay

Basic neuroscience assays are used to understand the structure and function of the brain and nervous system. These assays are used in research to study various aspects of brain function, such as neural development, synaptic transmission, and neurodegenerative diseases.

One common application is in the study of drug effects on the brain. Researchers use assays to determine how drugs interact with specific receptors or neurotransmitter systems in the brain. This information helps us to develop new treatments for neurological disorders and mental health conditions.
Another application is in the study of neuroplasticity, which is the brain's ability to reorganize itself in response to environmental or internal changes. Assays can be used to study how neural circuits are formed and how they change in response to learning, memory, or injury.
Researchers also use basic neuroscience assays to study the underlying mechanisms of neurodegenerative diseases such as Alzheimer's and Parkinson's disease. By studying the molecular and cellular changes that occur in these diseases, researchers can identify potential targets for treatment and develop new therapies.

FAQs

Q: What sample types do you accept for your assays?

A: We accept a wide variety of sample types for our neuroscience assays, including primary neuronal cultures, immortalized cell lines, brain tissue slices, and biofluids such as cerebrospinal fluid and blood. If you have a specific sample type or are unsure if your samples are suitable, our scientific team is available for consultation to discuss your needs and provide guidance on sample preparation and submission.

Q: Can you accommodate high-throughput screening for large-scale studies?

A: Yes, we are equipped to handle HTS for large-scale studies. Our laboratory is outfitted with automated systems that allow for the rapid processing of large sample volumes. This capability is essential for drug discovery and large-scale genetic studies. Our HTS services are designed to deliver fast, reliable, and reproducible data, making it easier to identify potential targets or compounds.

Q: Are your services suitable for both academic and commercial research?

A: Absolutely, our services cater to both academic and commercial research needs. Whether you're an academic researcher looking to explore fundamental neuroscience questions or a commercial entity involved in drug discovery and development, our services are designed to support your objectives. We offer flexible solutions and customized approaches to ensure our services align with your specific research goals and requirements.

Q: What are the costs associated with assay services?

A: The costs of our services vary depending on the specific assays requested, the number of samples, and any additional customization needed. We provide detailed cost estimates after an initial consultation to understand your project requirements. Our pricing is competitive, and we strive to offer value through high-quality results and exceptional service. We also offer flexible pricing options for long-term collaborations and large-scale projects.

Scientific Resources

Molecular and Cellular Neuroscience

Morphology Research Methods in Neuroscience

References

Wang, W.; et al. Molecular tools for imaging and recording neuronal activity. Nat Chem Biol. 2019, 15(2): 101-110.
Shafer, T. J. Application of Microelectrode Array Approaches to Neurotoxicity Testing and Screening. Adv Neurobiol. 2019, 22: 275-297.
Moffat, J. G.; et al. Opportunities and challenges in phenotypic drug discovery: an industry perspective. Nat Rev Drug Discov. 2017, 16(8): 531-543.

For Research Use Only. Not For Clinical Use.