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Knock-In Neural Models Products

Introduction Types Advantages Applications FAQs Related Product Sections Product List

Introduction

To advance neurobiological insight and therapeutic development for neurological disorders, models exhibiting faithful recapitulation of human disease are indispensable. Conventional genetic modification approaches, including overexpression systems, frequently introduce experimental artifacts, thereby diminishing translational relevance. Addressing this critical gap, Creative Biolabs provides sophisticated Knock-In Neural Model Cell Products. These models, meticulously engineered using advanced gene-editing technologies such as CRISPR/Cas9, offer unparalleled physiological relevance and precision. Such tools significantly bolster research into neurodegenerative and neurodevelopmental pathologies, enabling investigations to transcend the limitations inherent in traditional systems. Discover how our knock-in neural models can accelerate your breakthroughs. Contact us for a custom consultation or quote.

You may also directly consult our comprehensive Product List for specific offerings.

Types of Knock-In Neural Models We Offer

Creative Biolabs provides a diverse portfolio of knock-in neural cell models, leveraging cutting-edge gene-editing technologies like CRISPR/Cas9. We can generate specific genetic modifications in a range of neural cell types, including:

Types	Description
iPSC-Derived Neural Models	Neurons (e.g., cortical, motor, dopaminergic), astrocytes, oligodendrocytes, and microglia with your specified gene knocked in. We also engineer patient-derived iPSCs, performing corrective knock-ins or introducing reporters.
Immortalized Neural Cell Lines with Knock-Ins	Reliable and scalable models for high-throughput screening and long-term studies, engineered with specific gene insertions.
Primary Neural Cells with Custom Knock-Ins	While more complex, we can discuss the feasibility of introducing genetic modifications into primary neural cultures for highly specific applications.
Reporter Gene Knock-Ins	Insertion of fluorescent proteins (e.g., GFP, RFP), luciferase, or other tags at specific loci to track gene expression, protein localization, and cellular dynamics in live neural cells.
Specific Mutation Knock-Ins	Introduction of point mutations, SNPs, or small indels identified in human neurological diseases to create highly relevant disease models.
Humanized Gene Knock-Ins	Replacement of an endogenous rodent gene with its human counterpart in rodent neural cells to study human-specific gene function or drug responses in an in vivo-like context.

Advantages of Choosing Creative Biolabs' Knock-In Neural Models

Partnering with Creative Biolabs for your knock-in neural model needs offers distinct advantages.

Unparalleled Precision

Our expert application of CRISPR/Cas9 and other gene-editing tools ensures precise insertion at the desired genomic locus, minimizing off-target effects.

Physiological Relevance

Study gene function and disease mechanisms in the context of specific human neural cell types, including those derived from iPSCs, offering a closer reflection of human biology.

Customization at its Core

We don't offer one-size-fits-all. We work with you to design and generate the exact model you need, from gene target and cell type to specific reporter tags or mutations.

Expertise in Neuroscience

Our team possesses deep knowledge in neural cell culture, differentiation, and neurological disease modeling, ensuring the quality and relevance of your cell models.

Rigorous Quality Control

All our knock-in neural cell lines undergo stringent quality control measures, including genomic validation (PCR, sequencing), expression analysis (if applicable), and sterility testing.

Accelerated Research

Save valuable time and resources by leveraging our expertise and ready-to-use or custom-developed models, allowing you to focus on your downstream research goals.

Comprehensive Support

From initial project design to post-delivery troubleshooting, our scientific team is here to support you every step of the way.

Applications: Powering Breakthroughs in Neuroscience

Our Knock-In Neural Model Cell Products are versatile tools for a wide array of research applications.

Applications	Description
Modeling Neurological Disorders	Create high-fidelity in vitro models of Alzheimer's disease, Parkinson's disease, Huntington's disease, ALS, epilepsy, autism spectrum disorders, and other channelopathies or synaptopathies by introducing disease-specific mutations.
Gene Function Studies	Elucidate the role of specific genes in neuronal development, differentiation, maturation, synaptic function, and overall neural network activity.
Drug Discovery and Development	Screen compound libraries for efficacy in correcting disease phenotypes or modulating target gene activity in a human neural context. Assess drug toxicity and neuroprotective effects.
Reporter Lines for Cellular Dynamics	Track protein localization, trafficking, degradation, and gene expression patterns in real-time in living neural cells using knocked-in fluorescent or luminescent reporters.
Uncover Survival/Neuroprotection Pathways	Use KO cells for relevant genes to identify pathways for neuronal resilience or death, valuable for testing neuroprotective strategies.
Validation of Genetic Variants	Investigate the functional consequences of single-nucleotide polymorphisms (SNPs) or other genetic variations identified in patient populations.
Proof-of-Concept for Gene Therapy	Test the efficacy and specificity of gene therapy strategies by correcting genetic defects or introducing therapeutic genes in relevant neural cell models.

A picture that presents gene editing and gene expression modulation. (OA Literature)

Fig.1 CRISPR-Cas9 for gene editing and gene expression modulation.¹

FAQs

What are the main advantages over transgenic overexpression models?
Knock-in models express the modified gene from its native promoter, ensuring physiological expression levels and regulation. This avoids artifacts common with overexpression, such as protein mislocalization, aggregation, cellular stress, and non-specific effects, leading to more biologically relevant data.
What types of genetic modifications can be introduced?
We can introduce a wide range of modifications, including single-nucleotide variants (SNVs), small insertions/deletions (indels), disease-specific mutations, fluorescent reporter tags (like EGFP), epitope tags (like HA or Flag), and even larger cassette insertions.
Can you create models for specific neurodegenerative diseases like Alzheimer's, Parkinson's, or ALS?
Yes, we can generate knock-in models carrying specific mutations associated with various neurodegenerative diseases (e.g., APP, PSEN1/2, MAPT for Alzheimer's; SNCA, LRRK2, Parkin for Parkinson's; SOD1, C9orf72, TDP-43 for ALS).
Are these models suitable for high-throughput drug screening?
Yes, particularly our iPSC-derived neural models can be scaled for high-throughput screening (HTS) applications, offering a more physiologically relevant system than many traditional HTS cell lines.
Do you offer custom model generation services if the specific model I need isn't listed?
Yes, a significant portion of our work involves custom model generation tailored to specific research requirements. Please contact us with your project details.

The complexity of the nervous system and its disorders demands models that offer the highest fidelity. Creative Biolabs' Knock-In Neural Model Cell Products provide the precision, physiological relevance, and reliability you need to make groundbreaking discoveries.

Contact us to discuss your project and learn how our advanced knock-in neural models can empower your research and accelerate the path to novel therapies for devastating neurological conditions.