Online Inquiry

PSEN1 Gene-Engineered Cell Model Products

Categories Features Advantages PSEN1 Mechanisms in AD FAQs Related Product Sections Product List

PSEN1 (or Presenilin-1) is the main worker in an enzyme called gamma-secretase. It chops up this protein, APP, right in the cell's outer layer. This chopping makes different versions of amyloid-beta (Aβ) protein, and one type, Aβ42, is especially bad because it hates water and clumps up easily. This clumping is a big step in forming those toxic fibers that then build up as damaging plaques in the brain. Even small groups of Aβ42 can mess with calcium levels in cells, push another protein (tau) to form tangles, and even trigger cell death. How PSEN1 is shaped, thanks to certain parts and key bits called Asp257/385, decides what it acts on. Trying to treat Alzheimer's by tweaking gamma-secretase to reduce the bad Aβ42 without causing other issues depends on really understanding PSEN1's structure. Brain connections weaken, brain cells die – figuring out this enzyme is key to stopping Alzheimer's.

Consequently, gene-engineered cell models are indispensable tools for elucidating PSEN1 function and the impact of disease-associated mutations. Creative Biolabs offers a diverse portfolio of PSEN1 gene-engineered cell model products to support research endeavors in this critical area. Explore our PSEN1 Gene-Engineered Cell Model categories below for detailed information, or directly access our comprehensive Product List.

Featured PSEN1 Gene-Engineered Cell Model Categories

PSEN1 Mutation Models: These models empower researchers to investigate the effects of specific PSEN1 mutations, such as PSEN1 A246E and PSEN1 L435F, on a spectrum of cellular processes, including amyloid processing, tau pathology, and neuronal function.
PSEN1 Knock-out Models: By abrogating PSEN1 protein expression, these models aid in elucidating the normal physiological function of PSEN1 and the downstream consequences of its loss.
PSEN1 Knock-in Models: These models enable the precise introduction of specific PSEN1 variants, thereby providing a physiologically relevant system for studying subtle alterations in PSEN1 function.
PSEN1 Tagged Cell Lines: Expressing PSEN1 with various tags (e.g., GFP, FLAG) facilitates a multitude of experimental approaches, including protein localization studies, interaction analyses, and biochemical assays.

Features of Our PSEN1 Gene-Engineered Cell Models

Creative Biolabs offers PSEN1 gene-engineered cell models with the following features:

Features	Descriptions
Precise disease modeling	Cell models accurately replicate specific PSEN1 mutations and their effects on Alzheimer's disease.
Tools for targeted drug discovery	Models are invaluable for drug screening, assessing efficacy in modulating PSEN1 processing, and evaluating their ability to reduce toxic amyloid-beta peptide production.
Mechanistic insights	Researchers can use these models to dissect how PSEN1 mutations contribute to Alzheimer's disease.

Advantages of Our PSEN1 Gene-Engineered Cell Models

Creative Biolabs offers PSEN1 gene-engineered cell models with the following advantages:

Unparalleled Depth, Flexibility, and Translational Relevance: Our PSEN1 cell models go beyond simple disease replication, capturing the nuances of PSEN1 dysfunction for more relevant research and insights for Alzheimer's disease treatment.
Support for Advanced Imaging Techniques: Our models are compatible with advanced imaging, like confocal and live-cell imaging, enabling researchers to visualize dynamic processes and protein interactions with precision.
Robust Platforms for High-Throughput Screening: We develop cell models for efficient high-throughput screening (HTS), allowing rapid and cost-effective evaluation of potential therapeutic compounds.
Custom Model Generation Services: We offer custom model generation to design cell models tailored to your specific research objectives.

A picture presents PSEN1 possible Pathways. (OA Literature)

Fig.1 Pathways, in which PSEN1 may be involved.¹

PSEN1 Mechanisms in AD

There's this protein called PSEN1, and it's a big deal when we're trying to understand how Alzheimer's disease (AD) takes hold. It's not just about sending signals inside our cells; it also pitches in with breaking down old proteins and keeping our nerve cells humming along as they should. Now, PSEN1 is actually a crucial piece of a bigger puzzle – something called the gamma-secretase complex. Its main gig there is to cut up another protein, the amyloid precursor protein (APP). When it does that, it creates different kinds of these things called amyloid-beta peptides, and one specific type, Aβ42, is really bad news for the brain. The general idea is that when this Aβ42 starts to accumulate, it throws off how our brain cells communicate, kind of pushes the brain to form those tangled messes you often hear about in AD, and sadly, it eventually leads to nerve cells dying off. Because of all this, really digging into how PSEN1 works within this whole messy process is absolutely vital if we want to figure out ways to effectively treat Alzheimer's.

PSEN1 Mechanism	Description
Amyloid Generation	PSEN1 cleaves APP to produce Aβ peptides. PSEN1 mutations can change gamma-secretase activity, increasing the production of neurotoxic Aβ42, which aggregates into amyloid plaques, a hallmark of AD.
Synaptic Dysfunction	Aβ42 accumulation disrupts neuronal communication. PSEN1 mutations may directly or indirectly affect synaptic protein levels and function, exacerbating this.
Tau Pathology	PSEN1 mutations are also implicated in tau protein's abnormal phosphorylation and aggregation, another key AD feature. The exact mechanism is unclear, but PSEN1 may alter the kinase and phosphatase activity involved in tau phosphorylation.
Neuroinflammation	Aβ plaques and PSEN1 mutations can trigger brain inflammation, further damaging neurons and contributing to AD progression.
Mitochondrial Dysfunction	PSEN1 mutations may impair mitochondrial function, decreasing energy production, increasing oxidative stress, and promoting apoptosis.

FAQs

What types of PSEN1 mutations can you model?
Well, pretty much anything you can think of! We can model those tiny little changes, like where just one letter in the DNA gets swapped out. But we can also handle bigger stuff, like when whole bits of the gene are missing or extra pieces get stuck in there. And yeah, even those really complex genetic rearrangements? We can tackle those too. Our gene-editing tech is super adaptable, so it's all about making it fit exactly what you need for your research. Seriously, if you're focused on a specific PSEN1 mutation, reach out – we'd love to talk about how we can help you out.
What cell types are available for PSEN1 gene editing?
Gene editing is available in diverse cell types, including human induced pluripotent stem cells (iPSCs), neuronal cell lines, and other relevant systems. We also accommodate user-specified cell lines. Interested parties should contact our team.
Are there models available to study subtle alterations in PSEN1 function?
Yes, our PSEN1 knock-in models enable the precise introduction of specific PSEN1 variants.
What are the advantages of using gene-engineered cell models over traditional models?
When you're trying to really understand how diseases work and test out new drugs, using cell models that we've actually tweaked genetically gives you a much better picture, way more accurate and closer to what's really happening in the body compared to the old-school models. They allow for precise control over the genetic background and the introduction of specific disease-causing mutations. Let us show you how our models can enhance your research outcomes.
How do your PSEN1 cell models compare to commercially available cell lines?
Our PSEN1 cell models are custom-engineered to your exact specifications, ensuring the highest level of accuracy and relevance to your research. We also provide comprehensive validation data and ongoing technical support, setting us apart from standard commercial offerings. Contact us for a detailed comparison.
Can these models be used to study the effects of specific PSEN1 mutations?
Absolutely! Our PSEN1 mutation models are specifically designed to let researchers dig into how different PSEN1 mutations mess with all sorts of things in cells. We're talking about how they affect the production of amyloid, how they might lead to those tau tangles, and even how they impact the normal job of neurons. So yeah, they're perfect for studying the specific effects of different PSEN1 changes.

Creative Biolabs is committed to providing cutting-edge tools and expert support to accelerate neural research. Don't hesitate to contact us for learning more about our PSEN1 gene-engineered cell model products. We have the confidence to help you achieve your research goals.