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Custom Brain Organoid Services
Introduction of Brain Organoid
Brain organoid, also known as a cerebral organoid, refers to an artificial miniature organ that resembles the brain in vitro. In general, brain organoids are generated from pluripotent stem cells using a three-dimensional rotational bioreactor. The human brain is an extremely complex system that contains a variety of neuron types. In this case, human brain study, especially neurodegenerative diseases, becomes extremely difficult. Compared with rodent animal models, brain organoids present more advanced cell composition, maturation, and tissue architecture, which are closer to the human brain. In summary, brain organoids play important roles in neurological research and disease modeling.
Before being used as a model to study disease and gene expression, a series of assays are required for evaluation. For example, transcriptome analysis has been used to simulate the pathology of brain organoids. The TUNEL test is an evaluation indicator of apoptosis in brain organoids. Recently, computational methods have also been used to optimize the culture process of brain organoids.
Fig.1 Cerebral organoid at the interface of in vitro and in vivo. (Chiaradia, 2020)
Classification of the Brain Organoid
Based on our advanced platforms, Creative Biolabs now provides custom brain organoid services, including forebrain organoid, cerebellar organoid, whole-brain organoid, as well as retinal organoid.
- Forebrain organoid - It is the largest area of the human brain composed of the thalamus, the hypothalamus, the limbic system, and the cerebral cortex. The forebrain is the important neural structure that relates to speech, cognition, and emotion. It is also implicated in a series of neuropsychiatric conditions such as autism spectrum disorder and epilepsy.
- Cerebellar organoid - Located at the lower back of the brain, the cerebellum is an important regulating center of exercise and also plays an important role in maintaining body balance. Some studies showed that the functional defects of different cerebellar neurons may result in cerebellar dysfunction.
- Whole-brain organoid - The brain is the most advanced part of the nervous system and consists of the left and right hemispheres. It is the organ of thinking, leading all activities in the body and regulating the balance between the body and the surrounding environment.
- Retinal organoid - Derived from human pluripotent stem cells (hPSCs), the retinal organoid is the three-dimensional structure that recapitulates the spatial and temporal differentiation of the retina. It has been served as an effective in vitro model for retinal development.
Human Pluripotent Stem Cells 
Cultivate Embryoid Bodies Induce Neuroectoderm Grow in Gel Droplet Spin Brain Organoids in Bioreactor Completed Brain Organoid Ready for Various Applications Fig.2 Schematics show the basic steps for the generation of the cerebral organoid.
Service Features
| Features | Concrete Implementation |
|---|---|
| Specialized Generation | Our service specializes in producing brain organoids from induced pluripotent stem cells (iPSCs). We produce a range of brain organoids, including cerebral organoids that mimic the entire brain, and organoids that mimic specific brain regions, such as the cortex, hippocampus, or midbrain. |
| Comprehensive Quality Control | The quality of our organoids is assured through extensive testing. This includes stereological analyses to assess organoid size and shape, immunostaining to evaluate the presence of specific cell types, and RNA sequencing to analyze gene expression patterns. |
| Genome Editing | We provide genome editing services to create specific mutations in the organoids. This can be useful for researchers interested in studying the effect of certain genetic alterations on brain development or disease progression. |
| Long-Term Storage and Shipping | We offer long-term storage of organoids in appropriate conditions to maintain their viability and biological characteristics. The organoids can be shipped globally, ensuring researchers worldwide can access our products. |
We develop customized solutions based on the needs of our clients. From designing novel organoid platforms to customizing experiments, we go the extra mile to ensure our clients achieve their research objectives.
Applications of Brain Organoid
- Tissue morphogenesis
- Migration assays
- Cell fate potential
- Clonal lineage tracing
- Cell replacement therapy
- Transplantation
- Cell-type specific genome assays
- Drug testing
- Research on differences between species
Creative Biolabs is one of the well-recognized experts who are professional in applying advanced platforms for a broad range of neurosciences research, now we provide the novel STEMOD™ neuroscience ex vivo models for our clients all over the world. We can enable you to free up your time for core work and projects. If you are interested in our services and products, please do not hesitate to contact us for more detailed information.
FAQs
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Q: Can these brain organoids be used for long-term studies?
A: Yes, brain organoids can be maintained for long-term studies. With proper care and culture conditions, they can be kept viable for several months, enabling longitudinal studies across various stages of neural development or disease progression. -
Q: Can you customize the composition of the brain organoids based on specific cell types or genetic profiles?
A: Yes, we can tailor the composition of brain organoids to include specific cell types or genetic profiles relevant to your research interests, enabling more targeted experimentation. -
Q: How do you ship the organoids?
A: We ship organoids in a manner that ensures their viability and integrity upon arrival. Typically, this involves shipping in a specialized safe container at a regulated temperature. We also provide instructions for proper care and maintenance upon receipt. -
Q: Can these organoids effectively model neurological diseases?
A: Yes, brain organoids can effectively model various neurological diseases. They offer advanced disease modeling, even enabling the study of complex diseases like Alzheimer's, Parkinson's etc., in a controlled environment. -
Q: Can you support the project if I need a large number of organoids?
A: Absolutely. We can facilitate bulk orders for organoids. Just ensure that you give us early notice about the volume you need so that we can accommodate your requirements comprehensively.
Scientific Resources
Reference
- Chiaradia, I.; Lancaster, M. Brain organoids for the study of human neurobiology at the interface of in vitro and in vivo. Nature Neuroscience. 2020, 23(12): 1496-1508.
For Research Use Only. Not For Clinical Use.