Custom Brain Spheroid

Introduction of Brain Spheroid

The brain spheroids, also known as 3-dimensional brain cultures or cerebral organoids, refer to spherical laboratory-grown human brain tissue with 4mm in diameter. Inside the brain spheroids, thousands of neurons communicate with each other with an electric pulse. As an impressive form of tissue culture, they can't generate emotions and thoughts. Since it is very difficult to connect a functioning circulatory system to the spheroids, it seems impossible in theory to make a larger and more developed sphere.

Fig.1 Assessment of cell localization, tight junction protein expression, and barrier properties in self-assembled spheroids.^{1, 3}

Brain Spheroid-Based Research

Recent studies showed that brain spheroids have been used for the study of oligodendrocytes and related diseases. Based on standard brain spheroid, some extra steps allow the generation of brain spheroids containing both neurons and oligodendrocytes. The custom spheroids can then be used for disorders research that impairs myelination and oligodendrocyte development. Firstly, the custom spheroids were treated with two FDA-approved drugs, then accelerated oligodendrocyte development and myelination can be observed. Next, the novel spheroids with genetic mutations in Pelizaeus-Merzbacher disease were generated. In this case, novel drugs can be discovered using these disease-mimicking brain spheroids.

Generation of Brain Spheroid

For different disease processes, Creative Biolabs can generate a variety of brain spheroids types for neurology and oncology research. The brain spheroid can be generated from stem cells derived from a sample of human hair or skin. Compared with 2-dimensional counterparts, 3-dimensional brain cultures are much more complex to better model the human brain in vitro. Equipped with a special sample holder and sensitive imaging detectors, 3D images of brain spheroids can be obtained without slicing them.

Fig.2 Schematics show the timeline of processes involved in minibrain development.^{2, 3}

Applications and Advantages of Brain Spheroid

• High-throughput screening for drug discovery and toxicity testing
• Provide alternatives to some animal testing
• Understand how the brain develops
• Allow imaging and visualization
• Reproducible and cost-effective
• Cell therapy validation

Creative Biolabs is one of the well-recognized experts who are professional in applying advanced platforms for a broad range of neurosciences research, now we provide the novel STEMOD™ neuroscience ex vivo models for our clients all over the world. We can enable you to free up your time for core work and projects. If you are interested in our services and products, please do not hesitate to contact us for more detailed information.

Services at Creative Biolabs

As a groundbreaking neuroscience biotechnology company, we offer premier custom brain spheroid services. Our services primarily involve the creation of 3D brain spheroids, which are sphere-shaped 3D cultures of brain cells derived from human induced pluripotent stem cells (iPSCs).

Services Features

Our custom brain spheroid services are at the forefront of brain research and development. We utilize advanced biotechnology methodologies and protocols in our spheroid creation, and we ensure the highest quality spheroids for your research.

• Customizable Services - We have the ability to design and analyze three-dimensional brain spheroid models. This includes choosing the specific cell types and ratios to incorporate in the model.
• Advanced Technologies: We incorporate state-of-the-art biotechnology methods, including iPSC technology, gene editing, and high-throughput screening technologies.
• High Quality Standards - Our brain spheroids are characterized by high cell viability, precision, and reproducibility, ensuring the highest quality standards.
• Multifaceted Analysis - We offer real-time kinetics of neurospheroid development and function using advanced imaging systems. Our service includes detailed morphological, physiological, and molecular examinations of the brain spheroids.

Applications

As a neuroscience biotechnology company offering custom brain spheroid services, we provide robust and advanced solutions for various areas in the research field, with applications including:

• Drug discovery and development
• Disease modelling
• Neurodevelopmental studies
• Regenerative medicine
• Toxicology studies
• Cognitive neuroscience

By offering a customizable and physiological relevant model of the human brain, our custom brain spheroid services provide researchers with an innovative platform to facilitate more accurate and efficient studies.

FAQs

• Q: What types of assays or experiments can be performed using your custom brain spheroids?
  A: Our custom brain spheroids can be utilized in a wide range of assays and experiments, including drug screening, toxicity testing, neural development studies, and disease modeling. These versatile models faithfully recapitulate in vivo brain architecture, making them suitable for various research applications.
• Q: Can you accommodate specific requirements for sourcing cell types or genetic modifications in custom brain spheroids?
  A: Yes, we can accommodate specific requirements for cell types and genetic modifications in our custom brain spheroid services. Whether it involves sourcing specialized cell lines or introducing specific genetic alterations, we work closely with researchers to fulfill their experimental needs effectively.
• Q: What level of scalability do your custom brain spheroid services offer?
  A: Our custom brain spheroid services are scalable to accommodate a wide range of experimental scales, from small-scale proof-of-concept studies to large-scale screening projects. We optimize culture conditions and workflows to ensure scalability without compromising the quality or consistency of results.
• Q: Can you ensure the reproducibility of results obtained using your custom brain spheroids?
  A: Yes, we prioritize reproducibility in our services by employing standardized protocols, quality control measures, and rigorous validation procedures. Our commitment to consistency and reliability ensures that researchers can trust the reproducibility of their experimental results when using our custom brain spheroids.

Scientific Resources

High-efficient Astrocyte Differentiation Technology

Understanding Epilepsy Using Ex Vivo Hippocampus Brain Models

References

1. Nzou, Goodwell, et al. "Human cortex spheroid with a functional blood brain barrier for high-throughput neurotoxicity screening and disease modeling." Scientific reports 8.1 (2018): 1-10.
2. Govindan, Subashika, et al. "Mass generation, neuron labeling, and 3D imaging of minibrains." Frontiers in bioengineering and biotechnology 8 (2021): 582650.
3. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only. Not For Clinical Use.