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Custom Neural Differentiation Service
Introduction of Neural Differentiation
Derived from early blastocyst embryos, human embryonic stem cells (hESC) are self-renewable cells with pluripotency. Under special culture conditions, neural cells can be generated from hESC including glial cells, functional neurons, and oligodendrocytes. To avoid other lineage cells as contaminants, the neural progenitor (NP) cell population is necessary to the neural lineage and has been served as an unlimited lineage-restricted cell source for therapeutic and research.
Due to the stochastic nature of spontaneously differentiating hESC, the use of certain factors is important to direct hESC differentiation specifically to neural lineage. Once the NP cells are generated, the expression of SOX2 will be maintained and other neuroepithelial markers are also beginning to express, such as SOX1, SOX3, Nestin, PSA-NCAM, and MUSASHI-1. What's more, the formation of neural rosettes is the morphologic marker of hESC differentiation to neural cells.
Fig.1 Directed differentiation
of human embryonic stem cells (hESC) by adherent or suspension (embryoid body, EB) culture models. (Dhara, 2008)
Directed Neural Differentiation of hESC
In general, there are two types of culture systems for directed neural differentiation of hESC, including adherent and suspension culture. In a prolonged culture, an embryoid body (EB) can form a multilayered structure and cavities filled with cystic fluid. In addition, a variety of signaling molecules, factors, and conditioned media have been used for directed neural differentiation.
- For example, stromal-derived inducing activity (SDIS) mediated neural differentiation presents an efficient method, but unknown stromal factors may be introduced.
- Retinoic acid (RA) plays important role in neural development and activity, RA-mediated neural differentiation occurs by activation of genes, such as NFkB, SOX1, SOX6, BRN2, PSEN1, MAP2.
- Bone Morphogenetic Protein (BMP) signaling promotes the generation of neuronal, and Fibroblast Growth Factor (FGF) signaling has been implicated in the induction and patterning of neural tissues.
- The Neurobasal medium is essential for the derivation and maintenance of NP cells.
Service Features
Our service opens up a multitude of possibilities, offering distinctive features that cater to your specific needs.
| Features | Concrete Implementation |
|---|---|
| Precision and Customization | Our proprietary technology allows us to differentiate your specific stem cells into a variety of neural cell types, including neurons, astrocytes, oligodendrocytes etc., according to your research needs. |
| High Purity and Yield | Using advanced isolation techniques, we ensure high purity and yield in neural cells generated to meet the demands of your rigorous biomedical research and decrease the chance of experimental variation brought by undesired cell types. |
| Cell Line Development | We offer valuable assistance in producing, validating, and maintaining neuronal cell lines tailored to your specific study or model system, enhancing reproducibility for long-term projects. |
| Advanced Genetic Modification | We provide state-of-the-art genetic modification solutions such as CRISPR Cas9 genome editing to create specific knockouts, knock ins, point mutations, or fluorescent tagging of desired genes within your differentiated neurons. |
| Accelerated Productivity | Our efficient differentiation protocols drastically reduce the time it takes to obtaina large number ofthe desired neural cells, moving your project from concept to reality faster. |
| Quality Assurance | We proudly adhere to the highest industry standards to ensure quality, reliability, and reproducibility. All cell lines are rigorously tested to confirm their identity, purity, stability, and absence of pathogens. |
| Competitive Pricing | We believe in providing top-quality services at affordable pricing. Our competitive pricing framework is designed to accommodate your budget without compromising on the quality. |
| Comprehensive Support | Our skilled team of experts provides full-cycle client support, from initial consultation to project endpoint, we assist you with tailored protocols, troubleshooting, and detailed reports keeping you informed and confident throughout the process. |
Applications
Our custom neural differentiation service has numerous applications in various fields, particularly in biomedicine and scientific research.
Neural Differentiation for Neuroscience Research
Neural differentiation can help in understanding fundamental neurobiological processes. For instance, studying how stem cells differentiate into specific types of neurons can provide insights into brain development and functionality. Neurons derived through neural differentiation can also be used in toxicology studies to understand how different materials or compounds affect neuronal health.
Neural Differentiation for Disease Modeling
Numerous neurological disorders, including Parkinson's disease, Alzheimer's disease, and Huntington's disease, have been modeled using neurons derived from patient-specific iPSCs. Such disease-specific cell models allow for the investigation of disease mechanics and the screening of potential therapeutic drugs.
Neural Differentiation for Disease Treatment
In recent years, NP cells have been served as a primary cell source for novel drug discovery against neurodegenerative diseases. Promising lead compounds can be screened for neural diseases treatment using cell types that the disease affects. The motor neuron phenotype derived from NP cells can be used to study spinal muscular atrophy. In addition, there are many receptors expressed on NP cells, such as G-protein coupled receptors, ion channels, and other drug targets. In this case, NP cells and their derivatives present great potentials for drug discovery and evaluation against multiple neurological diseases.
Creative Biolabs is one of the well-recognized experts who are professional in applying advanced platforms for a broad range of neurosciences research. Now we provide the novel STEMOD™ neuroscience ex vivo models for our clients all over the world. If you are interested in our services and products, please do not hesitate to contact us for more detailed information.
Reference
- Dhara, S.; Stice, S. Neural differentiation of human embryonic stem cells. Journal of cellular biochemistry. 2008, 105(3): 633-640.
FAQs
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Q: How quick is the turnaround for the custom neural differentiation service?
A: The time it takes for this service can vary greatly depending on the complexity of the project and the specific requirements of the client. We would generally provide an estimated timeline before the work commences. Typically, the differentiation process takes a few weeks. -
Q: What makes the custom service different from standard services?
A: Unlike standard services, our custom service focuses on manipulating neural cells in a custom manner based on the specific needs of the project or research. This allows for greater flexibility and innovation in the field of study. -
Q: What types of neural cells can you differentiate?
A: We can differentiate all types of neural cells, including neurons, astrocytes, oligodendrocytes, and other specific subtypes. The requested cell type will be customized based on your project requirements. -
Q: What kind of quality control measures do you employ?
A: We implement stringent quality control measures to ensure high-quality output. This includes constant monitoring of cell morphology, purity assessment using flow cytometry, and functional verification through electrophysiological analysis. -
Q: How is the customization process done in the neural differentiation service?
A: The process starts with a thorough discussion about your specific needs. We then design a customized neural differentiation protocol, select the appropriate pluripotent stem cell line, and differentiate the cells under controlled conditions according to the desired neural cell type. -
Q: Are the custom neural cells ready to use?
A: Yes, the custom neural cells we provide are fully differentiated and mature, ready for immediate application in your research. We ensure they are healthy, robust, and ideal for your specific study needs.
Scientific Resources
For Research Use Only. Not For Clinical Use.