With years of experience in neuroscience and our most devoted scientists, Creative Biolabs is your reliable partner to provide high-quality and customized reprogramming factors for your neuroscience research.
Reprogramming and Reprogramming Factors for Development of Cell Models
During the decades since its birth, iPSC technology has already shown potential for clinical applications and disease modeling. Therefore, deriving high-quality, comprehensively characterized iPSC in a scalable process is crucial for their use in translational applications. Ever since work on pluripotency induction was originally published, reporting the reprogramming of somatic cells to iPSCs by the ectopic expression of the transcription factors, high expectations regarding their potential use for regenerative medicine have emerged.
Reprogramming is initiated by changes in the chromatin structure and transcriptome of a differentiated state into a pluripotent-like state. The reprogramming factors spontaneously bind together to form an interconnected autoregulatory circuitry, triggering their core promoter genes and cooperating with other pluripotency-associated genes. Reprogramming aims to generate pluripotent cells from any cell that can be isolated from a patient, partially erase the somatic epigenetic program, and reactivate the machinery involved in pluripotency.
Many methods for generating integration-free iPSCs have been developed that involve the transient expression of reprogramming factors (such as Oct4, Sox2, Klf4, and c-Myc), including adenoviruses plasmids, transposons, Sendai viruses, synthetic mRNAs, and recombinant proteins. Due to its reliability and high efficiency, retrovirus-mediated transduction remains the most widely used method for delivering reprogramming factors. Genes coding for reprogramming factors delivered in this way is randomly and stably integrated into the genome and affect the process, the differentiation of iPSCs into mature cell types, and variability between different iPSCs lines from a single patient.
Fig.1 Illustration of the wave models of reprogramming. (Prasad, 2016)
Services at Creative Biolabs
The generation of iPSCs from differentiated mature cells is one of the most promising approaches in stem cell-based models and regenerative medicine. Somatic cells can revert to the pluripotent state through the expression of specific reprogramming factors. The identification and study of these factors have helped to provide insight into the mechanism of induced pluripotency. As a top-ranking provider in the neuroscience market, Creative Biolabs offers various research tools to deal with the urgent demands for your neuroscience research and cell model development. With years of experience and our most devoted scientists, we are confident to tailor the reprogramming factors production to meet your specific requirements.
Discoveries of somatic cell reprogramming have opened the possibility of creating iPSCs using innovative strategies. Creative Biolabs is your trusted partner to offer the custom production of reprogramming factors for our global clients. Please contact us for more information and a detailed quote. We look forward to discussing your inquiry and finding the best solution for your needs.
Prasad, A.; et al. A review of induced pluripotent stem cell, direct conversion by trans-differentiation, direct reprogramming and oligodendrocyte differentiation. Regenerative Medicine. 2016, 11(2), 181-191.