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GLUT4 Gene-Engineered Cell Models Products

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Glucose transporter 4 (GLUT4) is a pivotal protein mediating insulin-sensitive glucose assimilation in peripheral tissues such as skeletal muscle and adipose. It is exhibiting escalating recognition for its salient role within the central nervous system (CNS). This burgeoning appreciation carries implications for a diverse array of neurological processes and disease states. Our portfolio of GLUT4 Gene-Engineered Cell Model products furnishes investigators with robust tools to dissect these intricate interdependencies. Through the precise modulation of GLUT4 expression and functionality within defined cellular systems, we empower researchers to attain more granular insights into neuronal metabolic pathways, synaptic dynamics, and the pathogenetic mechanisms underlying neurological disorders. Please contact us for more details.

You can explore our GLUT4 model categories below, or view our Product List.

Featured GLUT4 Gene-Engineered Cell Model Categories

Our GLUT4 Gene-Engineered Cell Model product portfolio encompasses a comprehensive array of cellular platforms, meticulously engineered to address the multifaceted inquiries of contemporary neuroscience research and to facilitate a granular comprehension of GLUT4's pleiotropic roles within the CNS milieu. Specifically, we provide:

GLUT4 Mutation Cell Lines: This category encompasses cells expressing GLUT4 with specific mutations, such as those affecting glucose transport, protein trafficking, or protein-protein interactions. These models help scientists break down how GLUT4 works and find the important parts for what it does. Scientists can change the GLUT4 protein in very specific ways. And this helps them understand why GLUT4 might not work right in diseases and how that causes problems. GLUT4-E329Q is A mutation in a region conserved between GLUT1 and GLUT4 that inactivates transport function, effectively locking GLUT4 in an inward-facing conformation. This model is ideal for studying the transport-dependent functions of GLUT4.
GLUT4 Knockout Cell Lines: In these models, scientists have turned off the GLUT4 gene using special new tools. This lets them see what happens to cells when GLUT4 isn't there or is very low. These models are really helpful for figuring out what GLUT4 normally does. And they help us understand how not having enough GLUT4 can lead to problems in the brain.
GLUT4 Overexpression Cell Lines: These cell lines feature enhanced GLUT4 expression, enabling the investigation of GLUT4's effects under conditions of increased activity. By upregulating GLUT4, researchers can see how taking in more sugar changes how brain cells talk to each other. It also shows how it affects how well brain connections change and how strong cells are. These models are really useful for looking at how the body tries to make up for problems. And they can show if making GLUT4 work better could help treat diseases.
GLUT4 Reporter Cell Lines: To facilitate the visualization and tracking of GLUT4 dynamics, we offer cell lines expressing GLUT4 tagged with fluorescent proteins or other markers. These reporter cells let scientists watch exactly where GLUT4 is in the cell, how it moves around, and how it connects with other proteins as it's happening. These models are super useful for understanding how the cell controls how much GLUT4 it makes and what GLUT4 does when different things happen. For example, when insulin is around, when brain cells are active, or when there's something wrong with the cell.

Features of Our GLUT4 Gene-Engineered Cell Models

Our GLUT4 Gene-Engineered Cell Models stand at the forefront of cellular research tools, embodying several key features that ensure their reliability, precision, and physiological relevance:

Feature	Description
Precise Gene Editing	At the heart of our model generation process lies the utilization of advanced gene-editing technologies. This ensures accurate and stable GLUT4 modification, guaranteeing that the desired genetic alteration is introduced with unparalleled precision. This precision is critical for generating cell models that faithfully represent the intended GLUT4 expression or function.
Disease Relevance	Recognizing the importance of physiological context, our models are based on a diverse array of neuronal and glial cell types, including but not limited to hippocampal neurons, cortical neurons, astrocytes, and microglia. We provide researchers with systems that closely mimic the in vivo environment, enhancing the translational potential of their findings.
Functional Validation	To make sure everything is scientifically correct, we check each cell model very carefully. This involves looking at it in a few different ways, like using techniques called Western blotting, qPCR, and immunofluorescence. We use these methods to make sure the gene changes we wanted actually happened. And we also use them to carefully see how those changes affect how much GLUT4 the cell makes and how well it works.

Advantages of Our GLUT4 Gene-Engineered Cell Models

Our GLUT4 cell models have several benefits. And these benefits let researchers explore the limits of brain research:

Enhanced Disease Modeling: By providing more accurate and physiologically relevant systems, our models enable researchers to more effectively recapitulate the cellular phenotypes associated with neurological diseases involving GLUT4 dysfunction. This helps us understand how diseases work better. And it helps us find new things to target to treat those diseases.
Improved Assay Development: The reliability and consistency of our gene-engineered cell models make them ideal for the development of robust and reproducible assays, facilitating high-throughput screening of potential therapeutic compounds and accelerating the translation of research findings into clinical applications.
Mechanistic Insights: Our models provide invaluable tools for dissecting the intricate molecular mechanisms that underlie GLUT4's role in critical neuronal processes, including synaptic signaling, plasticity, and the complex pathways involved in neurodegeneration.
Reduced Variability: Compared to primary cell cultures, which can exhibit significant heterogeneity, our gene-engineered cell models offer greater homogeneity. This reduced variability enhances the statistical power of experiments and improves the reproducibility of results, a cornerstone of scientific progress.

A picture presents the involvement of glucose transporters. (Koepsell, et al., 2020) (OA Literature)

Fig.1 Involvement of glucose transporters and a glucose sensor in d-glucose sensing by neurons that are excited by d-glucose (GE neurons).¹

GLUT4 Gene: Mechanisms and Principles

GLUT4 is a glucose transporter that plays a critical role in regulating glucose homeostasis. Please have a closer look at its mechanisms and principles:

Insulin-Regulated Glucose Transport: GLUT4 is primarily responsible for insulin-stimulated glucose uptake into cells, particularly in muscle and adipose tissues. In response to insulin signaling, GLUT4 translocates from intracellular vesicles to the plasma membrane, where it facilitates the transport of glucose into the cell.
Cellular Energy Homeostasis: By controlling glucose uptake, GLUT4 helps maintain cellular energy balance and supports essential metabolic processes.
Role in Disease: Dysregulation of GLUT4 expression or function is implicated in the pathogenesis of diseases like type 2 diabetes and Alzheimer's disease.

FAQs

Do your GLUT4 overexpression cell lines express the protein at physiological levels?
We can control the level of overexpression to mimic physiological or pathological conditions.
What mutations are available in your GLUT4 mutant cell lines?
We offer common mutations that affect glucose transport, protein trafficking, or protein interactions, and can also generate custom mutations.
Can you create a GLUT4 knockout in a specific patient-derived iPSC line?
Yes, we offer services to generate GLUT4-modified cell lines from customer-provided iPSCs.
Is it feasible to procure a cell line expressing GLUT4 conjugated with green fluorescent protein (GFP)?
Indeed, a selection of fluorescent moieties, including but not limited to GFP and red fluorescent protein (RFP), is available for conjugation with GLUT4 within our cell line offerings.
Do you provide cell culture protocols for your cell lines?
Yes, we provide detailed cell culture protocols to ensure optimal cell growth and performance.

Creative Biolabs' GLUT4 cell models give you a strong and trustworthy way to do your research. We have new technology and can change things to fit what you need. Plus, our experts are here to help you make new discoveries about metabolic diseases and how to make new drugs. If you want to know more about our GLUT4 cell models, just reach out to our team.