Neuronal Progenitors

• Sensory Neuron Progenitors Differentiation Service
• GABAergic Neurons Differentiation Service
• Dopaminergic Neurons Differentiation Service
• Cholinergic Neurons Differentiation Service
• Cerebral Cortical Neurons Differentiation Service
• Striatal Neurons Differentiation Service
• Motor Neurons Differentiation Service

Introduction of Neuronal Progenitors

Neuronal progenitors refer to the progenitor cells of the central nervous system (CNS) that produce glial and multiple neuronal cell types to populate the CNS. Generally speaking, the neuronal progenitors exist in the CNS of developing embryos, as well as the neonatal and mature adult brain. Based on their location, morphology, gene expression profile, time distribution, and functions in the brain, the neuronal progenitors present different characteristics.

Fig.1 Differentiation potential of neural progenitor cells derived from human embryonic stem cells. (Noisa, 2015)

Introduction of hES-NP Cells

With the ability to differentiate into different kinds of cell types, the human embryonic stem cells (hESCs) have been widely used to develop cell-based therapies against multiple neurodegenerative disorders, such as Huntington's disease, Alzheimer's disease, and Parkinson's disease. Some studies have shown that hESCs can be induced to neural progenitor cells by Dorsomorphin, which is a small molecule that inhibits BMP signaling. The obtained neural progenitor cells present neurobipolarity, high expression of neural progenitor genes, and multipotential differentiation ability.

Fig.2 Schematic of differentiation conditions for DA neurons from neural progenitors. (Noisa, 2015)

Based on the characteristics of stereotyping and proliferation, the hES-NP cells can be used as a scalable source to produce various types of neurons and glial cells, for toxicant and drug screening, and ultimately for cell-based alternative therapies. SHH and FGF8 are important dopaminergic (DA) neuron inducers, the DA neurons can be obtained from hES-NP cells following the exposure of SHH and FGF8. And its differentiation efficiency can be improved with LMX1A, a recombinant midbrain factor. In addition, better culture systems and microenvironments can further increase differentiation efficiency. The CBF1 and bFGF responsiveness showed that these hES-NPs are similar to embryonic neural progenitor cells. Besides DA neurons, a variety of neuronal subtypes can be obtained from hES-NP cells which include but are not limited as follow:

• Sensory neurons
• GABAergic neurons
• Dopaminergic neurons
• Cholinergic neurons
• Cerebral cortical neurons
• Striatal neurons
• Motor neurons

Characteristics of hES-NP Cells

• Stability in a long-term culture
• Ability to produce neurons and glia
• Expression of neural progenitor markers

Neuronal Progenitors in Developing Mammalian Neocortex

During embryonic neuro development, the proliferation of neuronal progenitors is largely regulated by a series of soluble factors, such as bFGF and EGF. These two factors are essential at different stages of brain development. Based on cell biology, there are three types of neuronal progenitors that exist in the developing mammalian neocortex, which include apical progenitors, basal progenitors, and subapical progenitors.

• Apical Progenitors (APs) - It has been described that there are three types of APs, including neuroepithelial cells, the derivative apical radial glia, and apical intermediate progenitors.
• Basal Progenitors (BPs) - There are two types of BPs distinguished as basal intermediate progenitors and basal radial glia, both of them can be obtained from APs or BPs themselves.
• Subapical Progenitors (SAPs) - Similar to BPs, SAPs undergo mitosis at an abventricular location.

Service Features

Creative Biolabs is one of the well-recognized experts who are professional in applying advanced platforms for a broad range of neurosciences research, now we provide the novel STEMOD™ neuroscience ex vivo models for our clients all over the world. If you are interested in our services and products, please do not hesitate to contact us for more detailed information.

At our company, we provide a comprehensive neuronal progenitor differentiation service that follows robust protocols to differentiate pluripotent stem cells into NPCs, which can further mature into various types of neurons.

• We begin by discussing the specific needs and requirements of the client's project. This includes the type of neuronal cells needed, the desired differentiation protocol, and any customization options.
• Clients can either provide their own neuronal progenitor cells or opt to use our established cell lines. We ensure the quality and purity of the cells to be used in the differentiation process.
• Our team of experts develops a customized differentiation protocol tailored to the client's specifications. This protocol includes the selection of growth factors, culture media, and other necessary components to induce the differentiation of progenitor cells into mature neuronal cells.
• We perform the differentiation process in our state-of-the-art laboratory facilities. The progenitor cells are cultured under controlled conditions, with regular monitoring and optimization to ensure efficient differentiation into the desired neuronal cell types.
• Throughout the differentiation process, we employ rigorous quality control measures to assess the purity, viability, and functionality of the differentiated neuronal cells. This includes immunocytochemistry, gene expression analysis, and functional assays to validate the identity and functionality of the cells.
• Once the differentiation process is complete and the desired neuronal cell population has been obtained, we deliver the mature neuronal cells to the client along with detailed documentation of the differentiation process and quality control results.
• We provide ongoing technical support to assist clients with any questions or issues that may arise following the delivery of the neuronal cells. Additionally, we offer follow-up services such as cell maintenance and expansion if needed.

Overall, our service offers a comprehensive solution for researchers and biotech companies seeking reliable and efficient generation of mature neuronal cells for their research and development projects. Perhaps you would also be interested in the following services.

Applications

• Neuroscience Basic Research
• Disease Modelling
• Drug Screening
• Cell Therapy
• Regenerative Medicine

FAQs

• Q: What types of neuronal progenitors can you differentiate?
  A: Our service covers the differentiation of various types of neuronal progenitors, including but not limited to cortical, hippocampal, and dopaminergic progenitors. We tailor the differentiation protocol based on your specific requirements and experimental needs.
• Q: Are your differentiated cells ready for use in genomic, proteomic, and functional studies?
  A: Absolutely. The differentiated neuronal cells that we generate can be ready for further multi-level analyses, which may involve genetic, protein, and synaptic function studies.
• Q: How can these differentiated neurons be transported to my laboratory safely?
  A: We have sophisticated packaging techniques to ensure cells remain viable and functional during transportation. These cells are packaged with specially-formulated media and packed to maintain a regulated temperature. We take utmost care to ensure prompt and efficient delivery to maintain the quality of the cells.
• Q: Can you provide after-service support in case of any issues or queries regarding the differentiated cells?
  A: Yes, we definitely provide after-service support. Our team of technical experts is here to help with any further queries or issues related to the differentiation of your progenitor cells into neurons. We rely on our technical expertise and research experience in the field to guide you throughout your experiment.

Scientific Resources

Emerging Tools for the Study of Neurological Diseases - Brain Organoids

Regulation and Drive of Neuronal Differentiation

References

1. Noisa, P.; et al. Neural progenitor cells derived from human embryonic stem cells as an origin of dopaminergic neurons. Stem cells international. 2015, 2015.
2. Florio, M.; Huttner, W. Neural progenitors, neurogenesis and the evolution of the neocortex. Development. 2014, 141(11): 2182-2194.

For Research Use Only. Not For Clinical Use.