ZfP521 Gene-Engineered Cell Models Products

A zinc finger protein ZfP521 plays an important role in regulating gene transcription in various tissues and is involved in neural differentiation, proliferation, and apoptosis. Aberrant ZfP521 expression has been implicated in several neurological conditions, making it a key target for disease modeling and therapeutic development. Our ZfP521 Gene-Engineered Cell Model Products offer cutting-edge tools for dissecting the intricate roles of ZfP521. Customers can expect delivery of cell models with ZfP521 gene overexpression, knockout, or specific mutations. These models enable precise investigation of ZfP521's impact on cellular function, signaling pathways, and disease progression.

You can explore our ZfP521 model categories below, or view our Product List.

Featured ZfP521 Gene-Engineered Cell Model Categories

We offer a variety of ZfP521 Gene-Engineered Cell Models, including:

• ZfP521 Mutated Cell Lines: ZFP521 mutated cell lines, engineered with specific gene alterations, serve as valuable tools for dissecting the multifaceted roles of this protein. The ΔZF27-30 mutation is a helpful tool for identifying specific protein-protein interactions that involve ZFP521. The Δ1-13aa mutation, on the other hand, provides valuable insights into ZFP521's role in epigenetic regulation through its interaction with the NuRD complex. Moreover, the K1146R mutation enables a focused investigation into the functional consequences of SUMOylation on ZFP521. This includes its precise cellular localization. Protein stability is also examined. Its activity within various cellular pathways is studied. These mutations are powerful research tools. They help us understand ZFP521 better.
• ZfP521 Knockout Cell Lines: These models feature the complete or partial removal of the ZfP521 gene, allowing researchers to meticulously investigate the functional consequences of its absence in various neural cell types. By observing how these cells change, you can find ZfP521's specific roles. This includes processes like neuronal development. Also, look at signaling pathways. Note cellular responses to stimuli.
• ZfP521 Knock-in Cell Lines: ZfP521 knock-in cell lines are precision-engineered models that integrate ZfP521 variants (wild-type or mutant) into defined genomic loci via site-specific recombination systems. These constructs usually combine regulatory elements, like inducible promoters, fluorescent reporters (T2A-mCherry), and selection markers for expression control. They are validated through sequencing and immunoblotting using anti-ZfP521 antibodies. These models help study the transcription factor's roles. The roles are in hematopoietic differentiation, bone development, and cancer progression. Applications include leukemia drug-resistance studies and high-content screening. This screening targets ZfP521-cofactor interactions.
• ZfP521 Reporter Cell Lines: These innovative models are engineered to express a reporter protein (such as a fluorescent protein like GFP or a luminescent enzyme like luciferase) under the control of the ZfP521 promoter or when ZfP521 is active. This capability enables the real-time visualization and quantification of ZfP521 expression and activity. Researchers can observe these changes in response to different stimuli. Drug treatments and various experimental conditions can also be monitored dynamically.
• ZfP521 Overexpression Cell Lines: Introducing extra copies of the ZfP521 gene into neural cells enables the study of the effects of increased ZfP521 expression. This is crucial for understanding potential gain-of-function mechanisms in disease and for identifying downstream targets that are sensitive to ZfP521 levels. These models can illuminate how ZfP521 contributes to neuronal function and the development of disease pathways when its expression is elevated.

Features of Our ZfP521 Gene-Engineered Cell Models

Advantages of Our ZfP521 Gene-Engineered Cell Models

• Accelerate Your Research: Our ready-to-use cell models save you valuable time and resources, allowing you to focus on your experiments.
• Generate Reliable Data: Our stringent quality control measures ensure the accuracy and reproducibility of your results.
• Gain Mechanistic Insights: Our cell models enable you to elucidate the precise role of ZfP521 in neural processes and disease pathogenesis.
• Facilitate Drug Discovery: Our models can be used to screen potential therapeutic compounds and identify novel drug targets.
• Access Expert Support: Our team of experienced scientists is available to provide technical assistance and guidance.

Fig.1 Zinc finger proteins (ZNF) associated with schizophrenia.¹

ZfP521 Gene: Mechanisms and Principles

ZFP521 encodes a protein with 30 Krüppel-like zinc finger motifs. These motifs are absolutely crucial for their role. It functions as a transcription factor. This allows it to bind to specific DNA sequences. As a result, ZFP521 can control the expression of a variety of different target genes within cells. ZFP521 carries out its regulatory roles through multiple distinct mechanisms. These mechanisms are quite varied. This intricate binding and regulation are key to its biological impact:

• Transcriptional Activation: ZFP521 can activate gene expression by recruiting co-activators such as p300 to target gene promoters.
• Transcriptional Repression: ZFP521 can also repress gene expression by binding to co-repressors like histone deacetylases (HDACs) through the NuRD complex.
• Protein-Protein Interactions: ZFP521 interacts with various other transcription factors, such as EBF1 and GATA-1, to modulate their activity and influence cell fate decisions.

FAQs

• What happens when ZFP521 expression is disrupted?
  Disruption of ZFP521 expression can halt neural differentiation and impair the development of the nervous system.
• What cell backgrounds are available?
  Creative Biolabs offers a diverse range of cell backgrounds. These include human and mouse cell lines. They also provide various neural cell types.
• Is technical support provided?
  Yes, Creative Biolabs provides comprehensive technical support and application notes.
• Can Creative Biolabs create custom cell models?
  Indeed, Creative Biolabs provides custom cell line engineering services specifically designed to meet unique research requirements.
• What is the significance of ZFP521 in drug discovery?
  ZFP521 is a potential therapeutic target, and cell models can be used to screen for drugs that modulate its activity.

Researchers gain advanced tools from Creative Biolabs' comprehensive ZFP521 Gene-Engineered Cell Model collection, enabling investigation into this protein's complex roles across biological processes, with a particular focus on neuroscience and neurological diseases. Our cell models are designed meticulously to provide accurate, reliable, and consistent results. This ensures your experiments yield trustworthy data. Scientific knowledge benefits from this. We are committed to providing customers with the highest quality products and services. You gain access to cutting-edge research tools. Expert support is always available. Contact us now. Explore how our models can significantly advance your research. Groundbreaking discoveries and innovative treatments are potential outcomes. Achieve more fluent research with our advanced cell models.

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Reference

1. Bu, Siyuan, et al. "Zinc finger proteins in neuro-related diseases progression." Frontiers in neuroscience 15 (2021): 760567. Distributed under Open Access License CC BY 4.0, without modification.