Previous research has demonstrated that the replacement of neurons in the mammalian CNS, lost to trauma or neurodegenerative disease, requires fetal tissues to be utilized. While this approach has been successful in the treatment of Parkinson's disease and has applied to Huntington's disease, and spinal cord injury, the ethical and logistical issues involved in obtaining enough donor tissue of appropriate age and region make the widespread therapeutic use of this transplantation strategy extremely limited. As such, many laboratories have investigated alternative tissues and/or cells for use in transplantation experiments to replace CNS neurons. Immortality is established when a cell loses its cell cycle checkpoint pathways. The overriding of natural cellular senescence takes place when the inactivation of p53/p16/pRb occurs during immortalization protocols. The mechanism controlling cellular senescence and immortalization was described as a two-stage mechanism, according to which, telomerase activity is a key factor in the establishment of immortality.
Immortalized neural cell lines have provided novel and important insight into fundamental principles of developmental neurobiology. These cell lines maintain the capacity to differentiate into neurons, astrocytes, and oligodendrocytes despite continued passage in cell culture, a property that is evidenced both in vitro and in vivo. Many of these cell lines differentiate after engraftment into the CNS with morphological and functional properties indistinguishable from endogenous neurons and glia.
Tissue source | Cell line | Immortalizing protein | Cell lineage in vitro |
---|---|---|---|
E14 mouse hypothalamus | Ht9-C7 | T-ag | primitive neurosecretory cell |
E7 quail neuroretina | QNR/D | ts-RSV | amacrine/ganglion cell (GABA) |
P2 rat cerebellum | ST15E, M15B | ts-T-ag, neu | astrocyte/neuron |
E10 mouse mesencephalon | 2.3D, NZen5-NZen37 | c-myc, N-myc | neuroepithelial precursor astrocyte/neuron |
Pl mouse olfactory bulb | OLB13-OLB24 | avian myc | neuron/oligodendrocyte |
P4 mouse cerebellum | C17.2, C27-3 | avian myc | neuron/oligodendrocyte astrocyte / neuron / oligodendrocyte |
Pl mouse striatum | SVLT.3.8 | T-ag | astrocyte/neuron |
E16 rat hippocampus | HiB5 | ts-T-ag | neuroepithelial precursor |
E17 rat hippocampus | H19-7, WH19-4 | ts-T-ag | astrocyte/neuron |
E13 rat medullary raphe | RN33B | ts-T-ag | neuron (glutamate) |
E14 rat striatum | M213-20, M26-1F | ts-T-ag | neuron (GABA) |
E7 mouse hippocampus | MK31 | ts-T-ag | neuron |
El8 rat hippocampus | HMR10-3 | ts-T-ag | neuron |
El3 rat medullary raphe | RN46A | ts-T-ag | neuron (serotonin) |
P5 mouse cerebellum | GC-B6 | ts-T-ag | astrocyte/neuron |
E14 rat striatum | ST14A | ts-T-ag | neuroepithelial precursor |
E12 rat mesencephalon | IRB3 A-N27 | ts-T-ag | astrocyte/neuron (dopamine) |
E14 rat mesencephalon | CSM14.1.4 | ts-T-ag | astrocyte/neuron (dopamine) |
Adult rat neuronal precursor cells | HC2S2 | v-myc | neuron |
E14 mouse hippocampus | MHP36 | ts-T-ag | astrocyte/neuron |
13-week human whole brain | B4, C2, CIO, JA3 | v-myc | neuron astrocyte/neuron |
15-week human telencephalon | H6 | v-myc | astrocyte/neuron/oligodendrocyte |
Table.1 Immortalized CNS neural cells lines.
Immortalized cell lines have become popular neuroscience research tools because of all the characteristics we mentioned above. As an industry-leading neuroscience research services provider, Creative Biolabs has been focusing on the application of immortalized cell lines in neuro over years. We are confident in offering quality-assured neuro-based immortalized cell lines custom productions. If you are looking for neuro-based immortalized cell lines custom productions or have any other inquiries about neuroscience research, please don't hesitate to contact us for more information.
Our services are designed to provide researchers with access to high-quality, reliable cell lines that can be used for a wide range of neuroscience applications. We aim to accelerate research and drug discovery in neurodegenerative diseases by rendering this service and related services, including but not limited to:
Services | Descriptions |
---|---|
Primary Cell Lines | We offer the development of neuroscience-based primary cell lines and related customized products. |
Custom Neural Differentiation | As experienced experts in neuroscience modeling, we offer comprehensive customized neural differentiation services to effectively support your neuroscience research. |
STEMOD™ Advanced Drug Discovery | We have developed a comprehensive technology platform to provide one-stop CNS drug discovery services. Our platform has advanced neuroscience ex vivo models, neuroscience assay techniques, and neuroscience research tools. |
Neruja Loganathan et al. reported that mHypoA-Ast1 cells, which represent a functional astrocyte lineage in the adult mouse brain, can be used to study the complex interactions of hypothalamic cells as well as the dysregulation that may occur in disease states, providing an important tool for neuroendocrine research.
They immortalized astrocytes from the hypothalamus of adult male CD-1 mice using SV40 T antigen to generate and characterize the mHypoA-Ast1 cell line. This cell line expresses astrocyte markers, responds to the inflammatory molecule lipopolysaccharide (LPS) and the fatty acid palmitate, and alters neuronal responses to palmitate.
This cell model can be used as a support cell for hypothalamic neuronal cell culture or for the study of hypothalamic-specific astrocyte function and disease.
Fig. 1 qRT-PCR analysis and immunocytochemistry of mHypoA-Ast1.1
Chronic neuropathic pain is a severe complication for much spinal cord injury (SCI) patients and the descending serotonergic system has been implicated in modulating nociceptive input. Using a model of chronic neuropathic pain in which the sciatic nerve is unilaterally constricted. Eaton and co-workers transplanted RN46A cells that had been genetically engineered to secrete BDNF into the intrathecal space of the lumbar spinal cord. BDNF expression in RN46A cells potentiates both their survival and serotonergic phenotype in vivo. Following these grafts, there was a reduction in tactile and cold allodynia as well as thermal hyperalgesia. The effects of these grafts could be blocked with the serotonergic antagonist methysergide, demonstrating that the effects were mediated by the released serotonin.
Immortalized neural precursor cell lines have proven effective in other models of CNS repair. The shiverer mouse suffers from widespread CNS demyelination because of dysfunctional oligodendrocytes. The meander tail mouse is characterized by a loss of cerebellar granule neurons. Synder and colleagues have examined the potential of engrafted C17.2 cells to repair these respective deficits. Following engraftment of C17.2 cells into the postnatal day 1 shiverer mouse ventricle, these cells myelinated roughly 50% of CNS axons, an extent of myelination that decreased tremor and restored near-normal motor function.
Q: Can your immortalized cell lines be used for high-throughput screening assays?
A: Yes, our immortalized cell lines are well-suited for high-throughput screening (HTS) assays. They offer the consistency and reproducibility necessary for such applications. We ensure that our cell lines exhibit stable growth characteristics and respond predictably to various stimuli, making them ideal candidates for drug screening, toxicity testing, and other high-throughput applications in neuroscience research.
Q: What customization options are available for your immortalized cell lines?
A: We offer several customization options to meet specific research requirements. This includes genetic modifications such as gene knock-in/knock-out, overexpression, or CRISPR/Cas9 editing. Additionally, we can adapt cell lines to express specific reporters or markers and modify culture conditions to suit unique experimental needs. Our team works closely with researchers to develop tailored solutions for their projects.
Q: What is the turnaround time for custom immortalized cell line development?
A: The turnaround time for custom immortalized cell line development varies depending on the complexity of the modifications required. Typically, the process can take anywhere from a few weeks to several months. We provide a detailed timeline and regular updates throughout the development process to keep researchers informed. Our goal is to deliver high-quality, customized cell lines efficiently to meet your research timelines.
Q: What are the pricing and ordering options for your immortalized cell lines?
A: Pricing for our immortalized cell lines depends on the specific cell line and any customizations required. We offer competitive pricing and bulk order discounts. Detailed pricing information and ordering options can be obtained by contacting our sales team or visiting our website. We strive to provide flexible purchasing options to accommodate the budgetary needs of different research projects.
Reference
For Research Use Only. Not For Clinical Use.