Neuronal Marker Antibody

Introduction of Neuronal Marker

The brain is the most complex organ in humans and nerve cells are the basic signal components of the brain. The central nervous system consists of a complex variety of neurons and glial cells, the glial cells can be divided into different categories, including astrocytes, microglia, and oligodendrocytes.

To have a better understanding of the brain's working mechanism, it is essential to study the different functions of various types of nerve cells. Morphologically, neurons are composed of four well-defined structural compartments, which include dendrites, axon, soma, and synaptic terminal. These highly compartmentalized structures allow us to distinguish them from other cell types. Now there are a series of proteins, also called neuronal markers, have been used for the identification of specific neuron subpopulations. In this case, the combination of neuronal marker antibodies with microscopy has been served as a powerful tool for multiple applications, which include but not limited to:

• Distinguish of cell types in the nervous system
• Phenotypic and morphological analysis of neurons
• Evaluation of cell or protein co-localization
• Visualization of synaptic connections
• Measurement of protein expression level
• Evaluation of cell health

Fig.1 Regional and cellular distribution of Hu expression in the adult avian neostriatum.

Types of Neuronal Markers

• Astrocyte markers - GFAP; Vimentin; S100B
• Microglia markers - IBA1
• Precursor cell markers - DACH1; Nestin
• Neuronal markers - TUBB3; MAP2; MAP1B; ELAVL3; ELAVL4; MAP1B
• Dopaminergic neuron markers - DOPA Decarboxylase; TH
• Oligodendrocyte markers - MBP; CNPase; Claudin 11; OLIG2
• Hippocampal CA1 marker - WFS1
• Glutaminergic neuron markers - GLS; GLAST; vGluT1; vGluT2; EAAT1; EAAT2; EAAT3; EAAT4; GAC; KGA; Glutamine synthetase; NMDAR1; NMDAR2B; GOT1
• GABAergic neuron markers - GAD65; ABAT
• Cholinergic neuron markers - CHT1; CHAT
• Axon markers - KIF5B; DYNC1H1

Fig.2 Schematic diagram of antibody structure.

Antibody Production Service at Creative Biolabs

Equipped with our advanced phage display platform, hybridoma platform, as well as other advanced antibody development platforms, we can provide a full range of neuronal marker antibody production services. From gene synthesis to antibody purification and labeling, we can produce antibodies in both research and diagnostic applications.

Creative Biolabs is one of the well-recognized experts who are professional in applying advanced platforms for a broad range of neurosciences research. If you are interested in our services and products, please do not hesitate to contact us for more detailed information.

Our neuronal marker antibody production services include the following key steps:

• Antigen selection: We work closely with our clients to identify and select the most appropriate antigens or target proteins that are specific to neuronal markers of interest.
• Antibody production: Our team of experienced scientists utilizes state-of-the-art technologies and techniques to produce custom antibodies targeting the selected neuronal markers.
• Antibody purification: Following antibody production, we employ advanced purification techniques to isolate and purify the desired antibodies.
• Antibody validation: Prior to delivery, all produced antibodies undergo rigorous validation processes to confirm their specificity and sensitivity for the target neuronal markers.
• Customization and optimization: We understand that different research projects may have specific requirements or preferences. As such, we offer customization and optimization services.

We also offer flexibility in our services, including but not limited to:

Published Data

Olig2 is an important transcription factor that is essential for the oligodendrocyte specialization and oligodendrocytes differentiation, as well as astrocytes and neurons during the developmental stage. Hui Wang et al. investigated the expression pattern of Olig2 in the CNS of adult mice by examining five major cell types, including OPC, oligodendrocytes, astrocytes, microglia, and neurons, in a series of studies to investigate the expression pattern of Olig2 in the adult CNS. OPC, oligodendrocytes, astrocytes, microglia and neurons, conducted a series of studies on Olig2 expression in the adult CNS.

They used rabbit anti-Olig2, mouse anti-S100β, mouse anti-CC1, mouse anti-CNPase, mouse anti-Olig2, and rabbit anti-NG2 primary antibodies for immunohistochemistry. All secondary antibodies were Alexa Fluor coupled antibodies. It was found that almost all OPC and oligodendrocytes expressed Olig2, no Olig2 signal was detected in microglia or neurons, and the expression pattern of Olig2 in adult astrocytes showed a region-specific pattern.

Fig. 3 The percentage of Olig2+ astrocytes in different mouse CNS regions.¹

Applications

Our antibodies are designed to specifically target and detect various neuronal markers, proteins, and antigens that are essential for studying neuronal structure, function, and development. Our neuronal marker antibody services are crucial for a wide range of neuroscience research applications, including:

• Immunohistochemistry (IHC) and immunofluorescence (IF): Our antibodies are used to visualize and localize specific neuronal markers within tissue sections or cultured cells.
• Western blotting: Our antibodies are utilized in western blotting assays to detect and quantify the expression levels of neuronal markers in cell lysates or tissue homogenates.
• Flow cytometry: Our antibodies can be used in flow cytometry assays to analyze and sort neuronal cell populations based on the expression levels of specific markers.
• ELISA: Our antibodies can be used in ELISA assays to quantify the levels of neuronal markers in biological samples, such as cerebrospinal fluid or blood plasma.
• Immunoprecipitation and immunoprecipitation-mass spectrometry (IP-MS): Our antibodies can be utilized in immunoprecipitation assays to isolate and identify protein complexes or interactions involving neuronal markers.

We collaborate closely with academic laboratories, pharmaceutical companies, and biotech firms to support their research endeavors and facilitate scientific discoveries in the field of neuroscience.

FAQs

Q: Can you assist me with selecting the best neuronal marker antibodies for my research project?

A: Yes, our expert team is available to consult with you on selecting the most appropriate neuronal marker antibodies for your study. We consider your specific research goals, sample types, species compatibility, and the detection method you plan to use. Based on this information, we recommend antibodies with the highest performance in similar applications, helping you achieve accurate and reliable results in your experiments.

Q: How long does it typically take to complete an antibody service project?

A: The timeline for our neuronal marker antibody services varies depending on the complexity of the project. For standard antibody production, it can take 3-6 months, depending on whether it's monoclonal or polyclonal. Custom antibody development, including antigen design and optimization, might require additional time. We work closely with clients to ensure timely project delivery while maintaining the highest quality standards throughout the process.

Q: What species are your neuronal marker antibodies compatible with?

A: Our neuronal marker antibodies are validated for use in multiple species, including human, mouse, rat, and various other model organisms commonly used in neuroscience research. If you require antibodies for less common species, we can optimize or develop custom antibodies specifically tailored for those applications. We provide detailed species compatibility information for each antibody to ensure reliable performance across your experimental models.

Q: Are your neuronal marker antibodies suitable for high-throughput screening?

A: Our antibodies are rigorously validated and optimized for various applications, including high-throughput screening. Whether you need antibodies for multiplex assays, large-scale screening, or automated imaging systems, we can provide options with high consistency, reproducibility, and batch-to-batch reliability. We also offer bulk supply and custom packaging to support large-scale experimental setups.

Scientific Resources

Neuronal Marker

Reference

1. Wang, Hui, et al. "Region-specific distribution of Olig2-expressing astrocytes in adult mouse brain and spinal cord." Molecular Brain 14 (2021): 1-14.

For Research Use Only. Not For Clinical Use.