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SOD1 Gene-Engineered Cell Models Products

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SOD1 is an enzyme found everywhere in cells. It is very important for protecting cells from damage caused by oxidation. It does this by changing harmful superoxide radicals. ALS is a terrible disease that damages nerves. It causes the loss of certain nerve cells that control muscles. This leads to muscle wasting and paralysis. Changes in the SOD1 gene are a major reason for ALS. Our specially made cell models are a strong tool. They help us understand the complicated ways this disease works.

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Featured SOD1 Gene-Engineered Cell Model Categories

At Creative Biolabs, we offer a diverse range of SOD1 Gene-Engineered Cell Model Products, categorized based on:

SOD1 Mutation Models: These models contain specific SOD1 gene alterations, mimicking ALS-patient mutations. These models are key to understanding how these mutations change what the protein does, where it goes, and how it sends signals. Scientists use them to study the specific ways that mutations show up. These ways include protein clumping, too much stress from oxidation, and problems with mitochondria. These models help show the detailed steps of how the disease gets worse. They let scientist study how mutant SOD1 interacts with other things in the cell. These things are helper proteins, enzymes that deal with redox, and proteins that make up the cell's structure. For example, A4V, G93A, and H46R are specific SOD1 mutations that can be modeled. Each of these mutations is linked to different ways ALS shows up in patients and how fast it gets worse.
SOD1 Knock-in Models: Our SOD1 knock-in models are powerful tools. We precisely introduce SOD1 genes into specific cells. This allows you to directly observe the protein's behavior. You can study its function in both normal and disease states. By using our models with mutated SOD1 genes, you can understand how these mutations impact the protein. This helps reveal how the disease process starts in ALS. Choose our models for your crucial ALS research.
SOD1 Knock-out Models: In these models, the SOD1 gene is inactivated, eliminating SOD1 protein expression. Researchers use them to see what happens when SOD1 is missing. They can study its role in ALS. Our knock-out models are key for understanding what SOD1 normally does. They also help study how the cell tries to make up for the loss, like making more antioxidant enzymes. You can also use them to find other targets for new drugs. Choose our SOD1 knock-out models for your important research.
SOD1 Overexpression Models: In these cell lines, the SOD1 gene is engineered to be overexpressed, meaning it produces significantly higher levels of the Superoxide dismutase 1 protein. These models help you study what happens when SOD1 activity is increased. This can give you insights into what SOD1 normally does in cells. It can also show possible ways to use SOD1 for therapy. Choose our models to learn more about SOD1's important roles.
SOD1 Tagged Cell Lines: Our tagged SOD1 protein lines are a great tool. We make SOD1 protein with a reporter tag, like GFP or FLAG. This helps you easily find SOD1, see where it is, and separate it out. With these lines, you can study how proteins interact with each other. You can also see where SOD1 is inside the cell. Plus, you can analyze how the SOD1 protein is changed after it is made. These lines are very useful for mapping groups of proteins. They help you find other proteins that connect with SOD1. You can also analyze how proteins move around in the cell. And you can purify groups of proteins to understand what SOD1 does in cell signaling.

Features of Our SOD1 Gene-Engineered Cell Models

Creative Biolabs' SOD1 Gene-Engineered cell models stand out for several reasons:

Feature	Description
Precise Gene Modification	Employing advanced gene-editing technologies to introduce specific SOD1 alterations with high accuracy, minimizing off-target effects.
Disease Relevance	Replicating key aspects of SOD1-linked ALS, closely mimicking the cellular and molecular changes associated with the disease.
Versatility	Offering a broad spectrum of models, including knockout, knock-in, and overexpression, to suit diverse research objectives and experimental designs.

Advantages of Our SOD1 Gene-Engineered Cell Models

Enhanced Model Relevance: Our gene-engineered cell models offer a significant advantage in accurately mimicking the complexities of human ALS. These models accurately mimic the gene changes and cell surroundings of the disease. Thus, they represent the disease more reliably than standard cell models. This better accuracy means research findings have a higher chance of leading to useful medical knowledge and treatment plans.
Increased Experimental Efficiency: The use of stable, well-characterized cell lines in our gene-engineered models streamlines experimental workflows and reduces variability. This consistency and reproducibility streamline research efforts. Investigators can depend on the stability of these models to generate robust datasets. Consequently, their focus can remain on fundamental scientific inquiries, thereby accelerating the pace of discovery.
Accelerated Research Progress: The reliability and accuracy of our SOD1 gene-engineered cell models empower researchers to obtain results more rapidly and with greater confidence. This accelerated pace of discovery enables faster progress in understanding the pathogenesis of ALS and in the development of novel therapeutic interventions. By providing a more efficient and effective platform for research, these models contribute directly to expediting the timeline for finding treatments and improving patient outcomes.
Complementary to In Vivo Studies: Our gene-edited cell models work well with animal studies. Animal studies show how ALS affects the whole body. But our cell models let you really zoom in. You can see the tiny details of what's happening inside cells in a clear and controlled way. When you use both our cell models and animal studies together, you get a much better picture of the disease. This can help find better and more specific ways to treat it.

A picture presents the ALS-associated SOD1 mutations. (Byström, et al., 2010) (OA Literature)

Fig.1 Structural positions of the ALS-associated SOD1 mutations. ¹

SOD1 Gene: Mechanisms and Principles

Think of SOD1 as a tiny but super important bodyguard inside your cells. Its job is to deal with harmful stuff called superoxide radicals – it's like neutralizing a tiny bomb by turning it into something harmless, oxygen and hydrogen peroxide. Now, if the instructions for making this bodyguard (the SOD1 gene) get messed up, you can end up with a bodyguard that's not shaped right and can't do its job. Even worse, this faulty bodyguard can actually cause trouble, setting off a chain reaction of bad events inside the cell.

The precise mechanism by which mutant SOD1 causes ALS is still not fully understood, but several key pathways have been implicated:

Oxidative Stress: Mutant SOD1 may lead to increased oxidative stress, damaging cellular components.
Protein Aggregation: Misfolded SOD1 protein can form toxic aggregates, disrupting normal cellular processes.
Mitochondrial Dysfunction: Mutant SOD1 can impair mitochondrial function, leading to energy deficits and increased oxidative stress.
Impaired Axonal Transport: Disruption of the transport of essential cell components along axons.
Excitotoxicity: Disruption of glutamate homeostasis, leading to overstimulation of neurons.
Endoplasmic Reticulum (ER) Stress: The accumulation of misfolded proteins in the ER.
Prion-like propagation: The spread of misfolded SOD1 protein from cell to cell.
Non-cell autonomous toxicity of neuroglia: The toxic effects of mutant SOD1 on surrounding glial cells.

Our gene-engineered cell models faithfully replicate these genetic alterations, allowing researchers to investigate these mechanisms and identify potential therapeutic targets.

FAQs

How do your SOD1 cell models compare to traditional ALS models?
Our gene-engineered cell models provide enhanced accuracy and reproducibility compared to traditional ALS models. Through precise introduction of specific SOD1 mutations, we mitigate the inherent variability associated with overexpression or random mutagenesis approaches.
Can I use your cell models for drug screening?
Yes, you can use our cell models for drug screening. We mainly focus on ALS caused by SOD1 gene changes. But our technology can also help study other forms of ALS. This includes sporadic ALS, which we don't fully understand. We can even look at how the normal SOD1 protein might play a role in the disease.
How do your cell models help in understanding the mechanisms of ALS?
Our cell models are like accurate copies of the gene changes in ALS. This lets scientists study the important ways the disease works. For example, they can look at oxidative stress. They can also study how proteins clump together. And they can investigate problems with mitochondria.
What is the cost of your SOD1 Gene-Engineered Cell Model Products?
The price changes. It depends on the exact cell model you need. It also depends if you want any special changes. And it depends on how many you order. Please contact us. We can give you a specific price quote. We can also talk about what you need for your project.
Is technical support available if I encounter problems using the cell models?
Yes, we offer comprehensive technical support to assist you with any issues.

Our SOD1 gene-engineered cell models are powerful tools for accelerating ALS research and the development of effective therapies. Contact us today to discuss your research needs in detail and learn how our cell models can revolutionize your work.