The human brain is the most complex organ that responds to all human intellectual abilities. In this case, a better understanding of human brain development is essential for the understanding of human cognitive abilities. In the past decades, transgenic animals are promising model organisms for study and research. However, there are significant structural differences between the brains of model organisms and humans. For example, increased proliferation of stem and progenitor cells as well as production of outer radial glial cells (oRGC) are primary mechanisms of primate cortical evolution, which is rare in rodents. In this case, brain organoids have been served as a powerful tool for human brain research in vitro. The forebrain is the important neural structure that relates to speech, cognition, and emotion. It is also implicated in a series of neuropsychiatric conditions such as autism spectrum disorder and epilepsy.
Fig.1 Validation of forebrain-type organoids at day 20. (Krefft, 2018)
Forebrain Organoid Culture System
To model human cerebral cortex development, there are telencephalic progenitor cells and neurons derived from a human pluripotent stem cell (PSC). However, PSC-derived 3D organoid cultures are required for the developmental processes of organogenesis. To avoid variations between batches and heterogeneity between organoids, now Creative Biolabs provides a simple and standardized forebrain organoid culture system. The most important feature and advantage of this system is the high efficiency and repeatability of PSC-derived organoids. As a stable and reliable cell-based model system, disease models can be generated in vitro.
Protocols for Forebrain Organoid Generation
The protocol for forebrain organoid generation is based on the combination of the self-organizing capacity of iPSC with their amenability to patterning factors. With the help of BMP (LDN-193189) as well as TGF-β type I receptor inhibitor (A83-01), individual-specific forebrain organoids can be obtained finally.
Generation of iPSC AggregatesInduction of Anterior NeuroectodermEmbedding of Neuroectodermal Aggregates in a Matrix ScaffoldGeneration of Forebrain-type Organoids from Neuroectodermal AggregatesFixation and Validation of Forebrain-type Organoids Fig.2 Schematics show the protocol for forebrain organoid generation.
Features of Forebrain Organoid Culture System
Repeatability to generate PSC-derived forebrain-specific organoids
Avoid the large variability between samples
Based on self-assembly without external control
Applications of Forebrain Organoid
Gene function studies
Creative Biolabs is one of the well-recognized experts who are professional in applying advanced platforms for a broad range of neurosciences research, now we provide the novel STEMOD™ neuroscience ex vivo models for our clients all over the world. We have the capability to enable you to free up your time for core work and projects. If you are interested in our services and products, please do not hesitate to contact us for more detailed information.
Krefft, O.; et al. Generation of standardized and reproducible forebrain-type cerebral organoids from human induced pluripotent stem cells. Journal of visualized experiments: JoVE. 2018 (131).