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APP Gene-Engineered Cell Model Products

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The Amyloid Precursor Protein (APP) functions as a vital component in Alzheimer's disease development. Precisely developed cell models provide crucial instruments for research on APP function and the effects of gene mutations related to diseases. Creative Biolabs offers a large variety of APP gene-engineered cell model products, enabling researchers to locate specialized tools for their specific research needs in preclinical studies and other applications. Learn more about our model categories below or jump directly to our Product List.

Featured APP Gene-Engineered Cell Model Categories

AD Mutation Models: Examine the specific impacts of variants such as APP Swedish (KM670/671NL), APP London (V717I), APP Indiana (V717F), and the APP N-terminal Val225Ala (V225A) mutation, which is associated with progressive dementia and influences tau protein interaction. Investigate how these mutations affect amyloid processing, tau pathology, and general cellular function.
APP Overexpression Models: Study the consequences of increased APP levels on cellular pathways relevant to AD, such as amyloid-beta production and synaptic dysfunction. Ideal for understanding dosage effects and downstream signaling.
APP Knock-Out Models: Elucidate the fundamental role of APP by examining cellular phenotypes in the absence of functional APP protein. Essential for understanding baseline functions and the impact of APP loss.
APP Knock-In Models: Investigate the subtle effects of specific APP variants or domains by precisely inserting them into the endogenous APP locus. Offers a more physiologically relevant context for studying specific alterations.
APP Tagged Cell Lines: Facilitate protein localization, interaction studies, and purification with our range of APP cell lines expressing tagged proteins (e.g., GFP, FLAG). Ideal for biochemical and cell biology assays.

A picture presents representative APP mutations. (OA Literature)

Fig.1 Representative APP mutations causing early-onset Alzheimer's disease with or without hemorrhage or stroke (in red).¹

Features of Our APP Gene-Engineered Cell Models

Creative Biolabs offers a sophisticated suite of APP gene-engineered cellular models, furnishing the research community with a robust platform for dissecting the multifaceted involvement of amyloid precursor protein (APP) in the pathogenesis of Alzheimer's disease (AD). These models offer a range of specific deliverables and solutions:

Features	Descriptions
Precise Disease Modeling	Cell models accurately replicate specific APP mutations and their associated effects on AD pathology, enabling more relevant research.
Targeted Drug Discovery	These models can be used to screen potential therapeutic compounds and assess their efficacy in modulating APP processing and Aβ production.
Mechanistic Insights	Researchers can utilize these models to study the detailed mechanisms by which APP mutations contribute to AD, leading to a deeper understanding of the disease.

Advantages of Our APP Gene-Engineered Cell Models

Creative Biolabs presents an augmented portfolio of APP gene-engineered cellular models, transcending conventional disease recapitulation to confer distinct advantages for the interrogation of Alzheimer's disease mechanisms.

Unlocking Deeper Insights: The unparalleled depth, flexibility, and translational relevance of our advanced cell models position Creative Biolabs as your indispensable partner for groundbreaking discoveries in Alzheimer's disease.
Extending Research Capabilities: Our models enhance research workflow and translational potential by supporting advanced imaging techniques like live-cell imaging and high-content screening through optimized tagged cell lines. They also streamline drug discovery and validation with robust platforms for high-throughput screening and comparative efficacy studies across diverse genetic backgrounds and disease stages. Moreover, bespoke model generation services are available, enabling investigators to procure cellular systems precisely tailored to specific research paradigms.
The Creative Biolabs Advantage: Choosing us means accessing cutting-edge innovation, expert scientific support, and a commitment to advancing AD therapeutics. Our advanced cell models offer unparalleled depth, flexibility, and translational relevance, making Creative Biolabs your indispensable partner for groundbreaking discoveries in Alzheimer's disease. Don't limit your research; choose Creative Biolabs.

FAQs

Do your AD Mutation Models exclusively focus on single-point mutations?
While our catalog features models harboring singular, well-characterized mutations, such as the Swedish and London variants, we possess the capability to generate or have in development models incorporating multiplex mutations or combinatorial genetic alterations involving APP and other genes implicated in Alzheimer's disease, thus facilitating the investigation of potential synergistic etiological mechanisms. Researchers are encouraged to consult our current portfolio and inquire about bespoke engineering services.
Can your APP Overexpression Models be utilized to investigate non-amyloidogenic APP processing pathways?
Notwithstanding their established utility in elucidating the generation of amyloid-beta peptides, our APP Overexpression Models also constitute a valuable platform for dissecting the impact of elevated APP expression on alternative processing cascades, notably the α-secretase pathway and the consequent formation of diverse APP-derived fragments potentially exhibiting distinct biological functionalities.
Are your APP Knock-Out Models suitable for the study of compensatory cellular mechanisms?
Affirmative. The examination of cellular phenotypes resultant from the absence of functional APP, facilitated by our Knock-Out Models, permits the identification of compensatory pathways or proteins that may undergo altered expression or activity to mitigate the loss of APP function.
Do your APP Knock-In Models permit the introduction of specific domain alterations beyond point mutations?
Indeed, our Knock-In technology affords the precise insertion of extended genetic sequences, encompassing entire APP domains or the creation of chimeric APP constructs. This capability enables granular analyses of individual APP domain function and its intramolecular interactions.
Can your APP-tagged cell lines be utilized for live-cell imaging of APP trafficking dynamics?
Affirmative. Our fluorescently tagged fusion protein systems (e.g., GFP/RFP variants) are expressly engineered for high-resolution, real-time visualization of APP spatiotemporal dynamics in situ. These models enable quantitative analysis of vesicular trafficking kinetics, subcellular localization shifts, and transient interactions with endogenous interactomes under physiologically relevant conditions.
Are species-specific or genetically tailored systems available upon request?
While our standardized repository prioritizes human immortalized lines (e.g., HEK293) with canonical genetic profiles, custom lineage development remains feasible. Proprietary CRISPR-Cas9-mediated genome editing, interspecies somatic cell hybridization, or induced pluripotent stem cell (iPSC) differentiation protocols may be deployed, contingent on target gene conservation, epigenetic compatibility, and phenotypic validation requirements. Submit experimental parameters for biotechnical assessment.
Does your platform accommodate novel APP mutations or post-translational modifications beyond catalog listings?
Synthetic pathobiology constitutes a cornerstone of our service architecture. Through iterative design-build-test cycles—incorporating site-directed mutagenesis, splice variant engineering, or inducible expression systems—we facilitate precise perturbation of APP isoforms. Collaborative workflows ensure fidelity to investigator-defined specifications, whether interrogating β-secretase cleavage susceptibility or modeling familial Alzheimer's disease-associated point mutations. Propose your construct; we operationalize it.
Are tiered pricing models accessible for large-scale or academia-driven initiatives?
A graduated cost structure aligns with institutional procurement frameworks. Academic consortia and high-throughput screening campaigns frequently qualify for non-linear price scaling. Contact our commercial division via templated RFQ portals for expedited quotation mechanisms, inclusive of MTA/MLA compliance documentation.

Creative Biolabs is dedicated to the provision of superior resources and comprehensive support to facilitate the progression of research into Alzheimer's disease. Investigators are invited to contact our team for detailed information regarding our APP gene-engineered cellular models and the potential for their customization to address specific research objectives.