High-efficient Astrocyte Differentiation Technology

A variety of neurological illnesses are linked to astrocyte dysfunction. Traditional research of astrocytes is based on the limited primary cells or mouse astrocytes. Astrocytes from human pluripotent stem cells are difficult and inefficient. Creative Biolabs, with well-established differentiation platforms, can rapidly and efficiently generate human astrocytes of high quality, regarding morphology, molecule profile, and function. We provide custom astrocyte differentiation services to our clients worldwide to facilitate your research.

Introduction

As a significant kind of cell in the mammalian central nervous system (CNS), astrocytes generate functional syncytia, participate in inter- and intracellular communication, and show optimal response to inflammation and damage, leading to the maintenance of brain homeostasis. With the confirmed overexpression protocol of transcriptional factor Sox9 and Nfib, iPSC-derived astrocytes are suitable for the research of astrocyte biology and neurologic physiology because of their similar phenotypes, molecule profiles, and functional properties to adult human astrocytes. The generated astrocytes are positive for astrocyte biomarkers such as GFAP, S100B, VIM, and ALDH1L1 following the decreasing marker expression of pluripotency and neural stem cells, and exhibit functional characteristics like the formation of gap junctions and synapse and cytokine-induced response. Moreover, iPSC-derived astrocytes perform well in mouse transplantation.

Custom Astrocyte Differentiation Service

Our technology shows good repeatability between batches and the induction to mature and functional astrocytes is time-saving (~3 weeks). The whole differentiation procedure involves tiny modifications in the genetic background of the parental cell line, which permits various strategies applied on this excellent platform, such as the genetic mutation-induced disease model and library screening to find the key factors mediating astrocyte differentiation. This stable system is reliable and trustable for your research involving disease development, drug discovery, functional examination, and transplantation trials to determine the interaction with the host neural system, together with the associated verification services like FACS, qPCR, and immunostaining to create more precise results.

Fig.1 CRISPR-modified cell line with mutations can be used for disease model research.

Highlights of Our Service

• High-quality and stable differentiation of astrocytes from iPSC and fibroblast.
• Thorough techniques and platforms to speed up your research.
• Well-established differentiation strategy with high repeatability between batches.
• Skillful scientific team to tailor your research strategy.
• Flexible scale of trials with low cost and short time.
• All-round comprehensive service for your project.

In the area of iPSC differentiation, Creative Biolabs has years of expertise in offering clients one-stop services. We build optimal platforms and well-trained teams dedicated to iPSC-derived astrocyte service and make them available to you. Don't hesitate to contact us for additional information.

Reference

1. Canals, Isaac, et al. "Rapid and efficient induction of functional astrocytes from human pluripotent stem cells." Nature methods 15.9 (2018): 693-696.

For Research Use Only. Not For Clinical Use.