SNCA Gene-Engineered Cell Models Products

Parkinson's disease, a neurodegenerative disorder of considerable importance, is fundamentally associated with the SNCA gene. This locus encodes the α-synuclein protein, and aberrant expression or processing thereof culminates in the pathognomonic intraneuronal inclusions known as Lewy bodies, a hallmark of PD neuropathology. Creative Biolabs provides a suite of SNCA gene-engineered cellular models and robust methodologies to enable granular mechanistic dissection of PD pathogenesis and accelerate the preclinical pipeline for innovative therapeutic interventions. Contact us for a detailed consultation to discover how our SNCA cell models can support your research and explore our customized solutions.

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Featured SNCA Gene-Engineered Cell Model Categories

We offer a variety of SNCA gene-engineered cell models, including:

• SNCA Mutation Models: Understanding exactly how specific SNCA mutations impact cellular processes is key. Our models allow researchers to have a close-up view of the way these mutations disrupt normal cell functions, from protein folding to behavior of the synaptic vesicles and the health of the mitochondria. For instance, the extensively researched SNCA mutations A53T, A30P, H50Q, G51D, V15A, and E46K have all been linked to distinct clinical presentations and the ways that the disease impacts the body.
• SNCA Knock-out Models: Elucidate the role of SNCA by removing its expression. SNCA knock-out models help determine the normal physiological function of α-synuclein. By completely removing SNCA expression, researchers can investigate the downstream effects on cellular signaling, synaptic plasticity, and neuronal survival and identify potential compensatory mechanisms.
• SNCA Knock-in Models: Carefully introduce SNCA variants for functional analysis. Knock-in models provide an advanced method for investigating SNCA variants in a context that reflects natural physiological conditions. In contrast to overexpression models, knock-in models entail the accurate incorporation of specific SNCA variants into their native genomic location. This ensures that the variant protein is produced at typical levels and is regulated in the same way as the wild-type protein.
• SNCA Tagged Cell Lines: Facilitate protein localization and interaction studies. Our SNCA-tagged cell lines express α-synuclein with tags like GFP or FLAG, enabling a multitude of experimental approaches to track protein movement, identify binding partners, and investigate protein complex dynamics.

Features of Our SNCA Gene-Engineered Cell Models

Creative Biolabs' SNCA gene-engineered cell models stand out for several reasons:

• Accurate Disease Modeling: Our cellular models meticulously mirror specific SNCA mutations' Parkinson-related impact, ensuring high fidelity to the disease state. This granular approach yields more reliable, translatable research outcomes by considering mutational effects on α-synuclein structure, function, and cellular interactions.
• Tools for Drug Discovery: Our cellular models serve as robust platforms for drug discovery, enabling the evaluation of therapeutic interventions targeting SNCA processing and mitigating cytotoxic protein aggregation. Designed to streamline the drug development pipeline, these models facilitate high-throughput screening and furnish critical data on compound efficacy and target specificity.
• Mechanistic Insights: These models aren't just about replication; they're designed to let you tease apart the complex ways SNCA mutations contribute to Parkinson's. We go beyond simply reproducing the disease phenotype. Our models enable you to delve into the underlying molecular mechanisms, unraveling the intricate pathways through which SNCA mutations exert their effects. This can lead to the identification of novel therapeutic targets and a deeper understanding of the disease.

Advantages of Our SNCA Gene-Engineered Cell Models

Creative Biolabs stands at the forefront of gene-editing technology, providing unparalleled expertise in the generation of custom cell models. Our SNCA Gene-Engineered Cell Model Products offer several key advantages:

Fig.1 SNCA variant p.V15A and well-known pathogenic variants p.A30P and p.A53T on the SNCA protein.¹

SNCA Mechanisms in PD

In order to appreciate the utility of our SNCA gene-modified cell models, one needs to grasp the function of SNCA within the context of Parkinson's disease. The SNCA gene encodes instructions for producing α-synuclein protein. Though its function is still being investigated, α-synuclein is believed to function at the synapses, or conduit sites, between nerve cells. In the disease, this protein misfolds and aggregates together to form Lewy bodies. These interrupt normal function of the brain and result in the characteristic motor and cognitive syndromes of PD.

Here's a more detailed look at the mechanisms involved:

• Protein Aggregation: A core feature of PD is the accumulation of misfolded α-synuclein. Certain mutations in SNCA can make this aggregation happen more readily.
• Synaptic Dysfunction: The buildup of α-synuclein disrupts the delicate machinery of the synapse, impairing communication between neurons.
• Neuronal Damage and Death: Ultimately, the dysfunction caused by α-synuclein leads to the damage and death of neurons, particularly in brain regions critical for movement control.

Understanding these mechanisms is crucial for developing effective therapies that target the root causes of Parkinson's disease.

FAQs

• What technical information is provided after purchasing your cell models?
  We provide detailed product datasheets that include the cell line's background, culture conditions, passaging methods, and relevant quality control reports.
• What cell types are available for SNCA gene editing?
  We offer gene editing in various cell types, including human-induced pluripotent stem cells (iPSCs), neuronal cell lines, and other relevant systems. We can also work with your preferred cell lines. Contact us to discuss your specific cell type requirements.
• Can you create models to study the effects of specific SNCA mutations?
  Yes, absolutely. Our SNCA mutation models are designed precisely for this purpose. They allow researchers to investigate how different SNCA mutations affect everything from protein aggregation to synaptic function and neuronal survival.
• How do your SNCA cell models stack up against commercially available cell lines?
  Our SNCA cell models are customized to meet your specific needs, guaranteeing exceptional accuracy and relevance to your research. Additionally, we offer thorough validation data and continuous technical support, which distinguishes us from typical commercial options. If you're interested in a detailed comparison, feel free to get in touch.
• Are your SNCA modifications verified?
  All our gene-engineered cell lines are subject to comprehensive molecular validation to ensure the correct genetic alteration.

Here at Creative Biolabs, we strive to supply you, the researcher, with the best quality SNCA Gene-Engineered Cell Model Products and professional services to speed up your research. Get in touch with us today to learn how we can adapt our models to suit your research needs. We are confident that we are capable of making your goals a reality.

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Reference

1. Diaw, Sokhna Haissatou, et al. "Characterization of the pathogenic α-Synuclein Variant V15A in Parkinson's disease." npj Parkinson's Disease 9.1 (2023): 148. Distributed under Open Access License CC BY 4.0, without modification.