GABAergic Neurons Differentiation Service

Overview of GABAergic Neurons

The amino acid G-amino-butyric-acid (GABA) is the major inhibitory neurotransmitter in the mature brain and plays an important role in regulating neuronal activity levels and synaptic plasticity, controlling the formation and propagation of neuronal activity, and generating local and large-scale oscillatory activity. In accordance with these diverse GABAergic functions, different classes of neocortical GABAergic interneurons with specific electrophysiological properties, dendritic morphologies, and axonal projection patterns have been identified and classified. In the mature midbrain, GABAergic neurons are found in several locations and participate in many important brain functions. Knowledge of GABAergic neuron development is highly important for understanding their diversity and function and may provide new approaches to the treatment of neurological and psychiatric disease.

Fig.1 GABAergic and glutamatergic domains and their gene-expression in the developing.¹

Diverse Mechanisms Regulate GABAergic Neuron Differentiation

No single transcription factor (TF) involved in patterning, neurogenesis or differentiation of GABAergic neurons is shared by all the GABAergic neuron populations. On the other hand, most of the TFs important for GABAergic neuron development also have functions in other neuronal lineages or cell types.

Services at Creative Biolabs

Focusing on custom neural differentiation models over years, Creative Biolabs has accumulated extensive experience and gradually established our own technology platform. During these years of exploration, we have trained a lot of experts specialized in these fields. With an excellent professional team, strong foundations, and rich experience, we are confident in offering customer-satisfied custom GABAergic neurons differentiation model service to global clients.

If you are interested in custom GABAergic neurons differentiation model service, or you any other models on our website, please don't hesitate to contact us for more information.

We can provide a wide range of services related to GABAergic neuron differentiation to support your research and development projects.

• In vitro GABAergic Neuron Differentiation
• Characterization of Differentiated GABAergic Neurons
• High Throughput Screening
• Gene Expression Profiling Services
• Customized Protocol Development
• GABAergic Neurons Viability and Proliferation Assays
• Training and Consultancy

We also offer flexibility in our services, including but not limited to:

Published Data

Ana Gonzalez-Ramos, et al. used a single-step approach to differentiate hESC into GABAergic neurons (hdIN) and test their function by overexpressing the transcription factors Ascl1 and Dlx2. After 35 days in vitro, hdIN showed electrophysiological properties and spontaneous synaptic currents comparable to those of mature neurons.

The electrophysiological properties of hdIN at different time points are shown in Fig 2. The differential cellular response to 0-25 pA depolarizing current ramps and 50 pA depolarizing current pulses at different time points is specifically demonstrated. These differences in intrinsic properties indicate that the function of hdIN matures significantly with time in culture.

Fig. 2 Electrophysiological properties of hdINs during the maturation process over time in culture.²

Applications

• High-quality cell cultures
• Disease modeling
• Drug discovery
• High-throughput screening
• Genetic engineering

FAQs

• Q: What techniques do you use to differentiate GABAergic neurons?
  A: We employ a combination of chemical induction and genetic manipulation techniques to drive the differentiation of pluripotent stem cells into GABAergic neurons. This involves carefully controlled exposure to specific signaling molecules and gene expression modulation to mimic the developmental processes that naturally occur in the brain.
• Q: How to address potential concerns regarding the presence of contaminated cell types in the differentiated neuronal population?
  A: We employ purification strategies such as selective media conditions, cell sorting techniques, and specific marker-based purification to enrich the desired GABAergic neuronal population and minimize contamination by other cell types. Additionally, we perform comprehensive characterization assays to confirm the purity and identity of the differentiated neurons.
• Q: Are costs broken down per step of differentiation?
  A: Generally, we provide a full-service package fee for the differentiation process. However, we are flexible and can discuss specific pricing structures based on your unique project requirements.
• Q: Can you provide post-delivery support, such as troubleshooting assistance or additional data analysis, after the completion of the project?
  A: Absolutely, our commitment to customer satisfaction extends beyond the completion of the project. We offer post-delivery support, including troubleshooting assistance, data analysis, and interpretation, to ensure the successful outcome of our customers' experiments. Our goal is to provide ongoing support and guidance to facilitate the advancement of scientific research using our differentiated neurons.

Scientific Resources

Signaling Pathways of Neuronal Differentiation

Regulation and Drive of Neuronal Differentiation

References

1. Achim, K., et al. Mechanisms regulating GABAergic neuron development. Cell Mol Life Sci. 2014, 71(8): 1395-415.
2. Gonzalez-Ramos, Ana, et al. "Human stem cell-derived GABAergic neurons functionally integrate into human neuronal networks." Scientific Reports 11.1 (2021): 22050.

For Research Use Only. Not For Clinical Use.