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Immortalized Cell Line Preparation Service
Introduction
Immortalized cell lines are essential for studying neuroinflammation and neurogenesis, as supported by neuroscience publications. Creative Biolabs provides immortalized cell line preparation via advanced technologies to establish stable, long‑term cell models. The service avoids phenotypic drift and ethical constraints, replacing unstable primary cultures with scalable, reproducible lines ideal for high‑throughput screening and translational CNS research.
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Immortalized Cell Line Preparation Service
This service introduces exogenous immortalization genes into primary cells via lentiviral or retroviral transduction. Through antibiotic selection, monoclonal screening, and functional validation, we establish stably subculture immortalized cell lines. The full workflow includes primary cell culture, vector construction, infection, selection, cloning, and QC characterization (telomerase activity, proliferation, phenotypic stability), delivering cell lines stable for at least 12 passages.
Core Advantages
- Break Proliferation Limitation: Overcome passage limits of primary neural cells, enable stable long-term culture (≥15 passages) without phenotype loss.
- High Genetic Uniformity: Stable genetic background and consistent traits reduce batch variation and improve data reproducibility.
- Customization & Compatibility: Support custom immortalization for multiple neural cell types and species, with hTERT, SV40 T antigen, etc.
- Strict Quality Validation: Full-scale QC ensures cell viability, phenotype, marker expression, and no contamination.
- Efficient Experimental Timeline: Shorten research cycles, lower costs, and enable large-scale high-throughput neuroscience studies.
Main Applications in Neuroscience Research
- Neurodegenerative Disease Modeling: Construct in vitro models of Parkinson's disease, Alzheimer's disease, ALS, and other diseases to explore pathogenesis and screen therapeutic drugs.
- Neural Cell Function Research: Study neuronal differentiation, axon growth, synaptic transmission, glial cell activation, and neuron-glia crosstalk mechanisms.
- Neurotoxicity & Neuroprotection Assays: Evaluate the neurotoxicity of chemicals and drugs, and verify the efficacy of neuroprotective agents.
- High-Throughput Drug Screening: Serve as stable cell models for large-scale screening of anti-neural disease and anti-glioma drugs.
- Gene & Cell Therapy Research: Act as ideal carriers for gene editing, gene overexpression/silencing, and lay a foundation for neural regenerative medicine research.
Common Immortalized Cell Lines for Neuroscience Research
| Cell Line | Cell Origin | Key Biological Features | Main Neuroscience Applications |
|---|---|---|---|
| PC12 | Rat adrenal pheochromocytoma | Responds to NGF stimulation to form neurites; differentiates into sympathetic neuron-like cells; secretes catecholamines | Neurite outgrowth assays, neurotrophic factor research, neurotransmitter release detection, and peripheral neuron models |
| Neuro-2a (N2a) | Mouse neuroblastoma | Fast proliferation, easy to transfect; readily induced to differentiate; retains neuronal morphology and signaling pathways | Neuronal differentiation mechanism, axon guidance, Alzheimer's disease model, gene overexpression/silencing studies |
| HT-22 | Mouse hippocampal neuron subclone | Hippocampal neuronal phenotype: sensitive to oxidative glutamate toxicity; no excitatory amino acid receptors | Oxidative stress in neurons, Alzheimer's disease, excitotoxicity, neuroprotection, and antioxidant drug evaluation |
| BV2 | Mouse microglial cell line | Retains classic microglial morphology and immune reactivity; activates in response to LPS/IFN-γ; mimics primary microglia function | Neuroinflammation, microglial activation, phagocytosis, neuroimmunology, neurodegenerative inflammatory mechanism |
| C6 | Rat glioma | Expresses glial fibrillary acidic protein (GFAP); typical astrocyte phenotype; stable passage | Astrocyte function, glial tumor modeling, glia-neuron interaction, glioma drug screening |
| U-87 MG | Human glioblastoma astrocytoma | Highly aggressive astrocytic tumor line; easy genetic modification; strong invasiveness | Glioblastoma pathogenesis, brain tumor invasion, anti-glioma drug development, targeted therapy research |
Workflow
The process of transforming primary tissue into an immortalized, research-ready asset is a multi-stage engineering feat designed for maximum stability.
Fig.1 The classic process for preparing immortalized cell lines.1,3
What We Can Offer
At Creative Biolabs, we don't just provide cells; we provide a complete technological ecosystem for your research. Our Immortalized Cell Line Preparation Service is a full-scale, one-stop solution tailored to the rigorous demands of biology experts and pharmaceutical innovators.
One-stop Immortalization Service
From initial primary tissue isolation and vector design to pilot-scale expansion and large-scale cryopreservation.
Customized Engineering Options
Precise modification of genes via CRISPR/Cas9 (KO/KI) or the integration of cell-specific reporters (GFP/Luciferase) to meet your unique experimental needs.
Optimized Codon Usage & Vector Selection
We optimize gene expression levels using a variety of viral delivery systems (Lentivirus, Retrovirus, Adenovirus) to maximize the stability of the immortalizing phenotype.
Guaranteed Stability & Purity
Rigorous assessment of strain/cell origin and stability across 50+ passages, ensuring your Master Cell Bank (MCB) remains phenotypically consistent.
Strict Aseptic & Quality Systems
Our processes follow the basic principles of Good Manufacturing Practice (GMP) and include strict aseptic verification throughout the cultivation and bank-building phases.
High-Standard Quality Control
Implementation of Quality-by-Design (QbD) and process analytical techniques (PAT) to quantify marker expression and ensure karyotypic integrity.
Scalable Production Capabilities
From lab-scale T-flasks to large-scale bioreactor expansion, providing over 100 billion cells for high-throughput screening campaigns.
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Case Study
Researchers aimed to establish an immortalized mouse spiral ganglion neuron (SGN) cell line. They infected primary P1 mouse SGNs with a lentiviral vector overexpressing SV40LT (LV-SV40), selected with puromycin, and obtained a monoclonal cell line named SIO-SGN1. Immunostaining showed successful SV40LT integration and altered neuronal morphology. Cell counting, cell staining, and Ki67/PCNA detection verified stable proliferation for at least 20 passages and retained contact inhibition, confirming a safe and stable immortalized SGN cell line.
Fig.2 Establish an immortalized mouse spiral ganglion neuron (SGN) cell line by overexpressing SV40LT in lentivirus.2,3
Customer Reviews
FAQs
Q: How do you ensure the immortalized line still behaves like a primary neuron?
A: We perform rigorous phenotypic characterization and compare marker expression against the original primary cells.
Q: What is the advantage of using hTERT over SV40 T-antigen?
A: hTERT is often preferred for human cells to avoid oncogenic transformation signatures.
Q: Can these cell lines be used for high-content screening (HCS)?
A: Yes, they provide the uniform cell density and predictable growth curves essential for automated imaging.
Q: Are there precautions regarding the use of viral vectors?
A: All lines are provided with safety documentation; BSL-2 practices are recommended.
Q: How do your custom lines compare to commercial 'off-the-shelf' versions?
A: Our custom service allows you to dictate exact donor characteristics and genetic modifications.
Contact Our Team for More Information and to Discuss Your Project
References
- Ke, Yankun, et al. "Establishment of an Immortalized Canine Hippocampal Neural Stem Cell Line via SV40LT Retroviral Transduction." Cells 15.6 (2026): 543. https://doi.org/10.3390/cells15060543.
- Wang, Xue, et al. "An immortalized cochlear spiral ganglion neuronal cell line: a promising tool for hearing loss study." Neuroscience (2025). https://doi.org/10.1016/j.neuroscience.2025.07.017.
- Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only. Not For Clinical Use.