What comes in the vial?

Each vial contains ≥ 1 million cryopreserved cells, a certificate of analysis (CofA) with QC data, and a detailed protocol for handling and culture.

What is the recommended seeding density?

This is application-dependent and is detailed in the product-specific protocol. For general culture, a density of 50,000-100,000 cells/cm² is typical.

Are these models suitable for studying genetic forms of hydrocephalus?

Yes. They provide an ideal background for CRISPR-Cas9-based gene editing to introduce specific mutations or can be used as controls for patient-derived cell lines.

Can I perform immunocytochemistry (ICC) on these cells?

Yes, the cells are amenable to standard fixation and staining procedures. Our protocols provide recommendations for optimal results.

Can I extract RNA/protein for omics studies?

Yes, the cells are an excellent source of high-quality biological material for transcriptomics, proteomics, and other molecular analyses.

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Hydrocephalus Cell Model Products

Introduction Types Advantages Applications FAQs Related Product Sections Product List

Introduction

Hydrocephalus is a complex neurological disorder defined by the abnormal accumulation of cerebrospinal fluid (CSF) within the brain's ventricles. This condition, resulting from an imbalance in CSF production, flow, or absorption, leads to increased intracranial pressure, ventricular enlargement, and potentially severe neurological damage. For decades, research has been hampered by the intricate pathophysiology and the challenges of studying cellular dynamics within the living brain. Traditional approaches have provided limited insight, creating a critical bottleneck in the development of novel, non-surgical therapies.

Creative Biolabs is closing that gap. As a leader in preclinical research solutions, we provide the scientific community with a sophisticated portfolio of high-purity, functionally validated cell models specifically designed to investigate the core cellular mechanisms of hydrocephalus. Our models empower researchers to dissect the complex interplay between the cells governing CSF homeostasis and neuroinflammation, enabling high-throughput screening, mechanistic studies, and the identification of promising new therapeutic targets. Move beyond the limitations of conventional models and accelerate your research with cell systems that offer unparalleled clinical relevance. Contact our team of experts to review your project needs and to explore our models or the design of a custom solution.

Quickly find the product you need by exploring our complete Product List.

Types

We provide a comprehensive range of cell models to suit diverse research needs, from high-throughput screening to complex mechanistic studies.

Types	Description
Mutant Lines	Featuring common hydrocephalus-associated mutations (e.g., in L1CAM, MPDZ, CCDC88C), these cell lines are fully validated and available for immediate shipment.
Isogenic Control Lines	For every mutant model, we offer the corresponding wild-type (WT) parental line, ensuring that observed phenotypes are directly attributable to the specific genetic modification.
2D Monolayer Cultures	Ideal for biochemical assays, compound screening, and molecular analysis, providing a scalable and highly reproducible format.
3D Spheroid/Organoid Models	These advanced models recapitulate tissue-like cytoarchitecture, enabling the study of complex cell-cell interactions, barrier function, and CSF dynamics in a more physiologically representative environment.
Custom-Engineered Models	We can generate models with any client-specified mutation in various relevant cell backgrounds.

Advantages

By integrating Creative Biolabs' models into your workflow, your research gains a significant competitive edge.

Accelerate Your Timeline

Bypass the time-consuming and resource-intensive process of de novo model development, which can take 6-12 months. Our models allow you to start your critical experiments in weeks.

Unparalleled Reproducibility

Eliminate the inherent variability of primary cell cultures or the complexity of animal models. Our isogenic cell lines provide a stable, consistent system for reproducible data generation.

Deepen Mechanistic Insight

Directly investigate the functional consequences of specific disease-causing mutations in a controlled cellular context.

Enable Effective Therapeutic Screening

Our models provide a robust and scalable platform for testing the efficacy and toxicity of potential therapeutic compounds early in the discovery pipeline.

Applications

Applications	Description
Disease Modeling	Investigate the cellular and molecular underpinnings of both congenital and acquired hydrocephalus.
Drug Discovery & High-Throughput Screening (HTS)	Screen compound libraries to identify novel drugs that restore ciliary function, reduce CSF production, or modulate neuroinflammation.
CSF Dynamics Research	Study the barrier function, secretory mechanisms, and fluid transport properties of the choroid plexus and ependymal lining.
Neuroinflammation Studies	Model the inflammatory response of glial and ependymal cells to stimuli relevant to hydrocephalus, such as hemorrhage or infection.
Ciliopathy Research	Directly investigate defects in ciliary structure and function (ciliogenesis, ciliary beating) associated with genetic forms of hydrocephalus.
Developmental Neurobiology	Explore the differentiation and maturation of ventricular-zone cell types and how these processes are disrupted in disease.

A picture that presents a Schematic of a choroid plexus cell. (Newland, et al., 2024) (OA Literature)

Fig.1 Schematic of a choroid plexus cell with the ion transporters that may be involved with hydrocephalus.¹

FAQs

What comes in the vial?
Each vial contains ≥ 1 million cryopreserved cells, a certificate of analysis (CofA) with QC data, and a detailed protocol for handling and culture.
What is the recommended seeding density?
This is application-dependent and is detailed in the product-specific protocol. For general culture, a density of 50,000-100,000 cells/cm² is typical.
Are these models suitable for studying genetic forms of hydrocephalus?
Yes. They provide an ideal background for CRISPR-Cas9-based gene editing to introduce specific mutations or can be used as controls for patient-derived cell lines.
Can I perform immunocytochemistry (ICC) on these cells?
Yes, the cells are amenable to standard fixation and staining procedures. Our protocols provide recommendations for optimal results.
Can I extract RNA/protein for omics studies?
Yes, the cells are an excellent source of high-quality biological material for transcriptomics, proteomics, and other molecular analyses.

Our hydrocephalus cell models are empowering research across the entire drug discovery pipeline. Whether you are validating a new target, screening a compound library, or elucidating the fundamental biology of the disease, Creative Biolabs provides the critical tools you need to generate meaningful, translatable data. Contact our scientific team to strategize the next steps for advancing your hydrocephalus research.