Can I co-culture different cell types from your portfolio, such as neurons and glial cells?

Yes, in fact, we highly recommend it. Co-culturing our neurons with glial cells (like astrocytes) creates a more physiologically relevant microenvironment. This is crucial for investigating neuro-glial interactions, inflammatory signaling, and modulatory factors that play a key role in TTH pathology. Please contact our technical support for advice on optimized co-culture media and protocols.

Are these primary cells suitable for genetic modification, such as transfection or viral transduction?

Primary neurons are sensitive and can be difficult to transfect with traditional chemical reagents. However, they are highly amenable to gene modification using viral vectors. We recommend using lentiviral or AAV-mediated transduction for efficient gene delivery while maintaining optimal cell health. Optimization of the Multiplicity of Infection (MOI) is advised for best results.

Do you offer bulk purchasing discounts or custom supply solutions for large-scale screening projects?

Absolutely. We understand the demands of high-throughput screening (HTS) and large-scale studies. We offer competitive pricing for bulk orders and can arrange custom, flexible delivery schedules to align with your project timelines. Please contact our sales department to discuss your specific needs and receive a customized quote.

Can I request a custom cell isolation?

We do offer custom services. Please contact our team with your specific requirements, and we can discuss the feasibility of your project.

Can these models be used to study medication-overuse headache?

Yes, they provide an excellent in vitro system to study cellular adaptations and sensitization following chronic exposure to analgesics.

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Tension Headache Cell Model Products

Introduction Types Advantages Applications FAQs Related Product Sections Product List

Introduction

For too long, the intricate pathophysiology of tension-type headaches (TTH) has been a "black box," limited by the lack of clinically relevant and reliable research models. Generic cell lines and animal models often fail to replicate the key mechanisms, from myofascial triggers to central sensitization, slowing down the discovery of effective therapeutics.

Creative Biolabs is changing that. We provide researchers with a specialized portfolio of high-purity, cryopreserved primary cells directly implicated in the TTH pain pathway. Our models offer a powerful in vitro platform to investigate molecular drivers, screen novel compounds, and generate translatable data. Contact our scientific team today to discuss your project needs, request a quote, or learn more about our custom model development services.

To find a specific product, please see our complete Product List.

Available Model Types

We offer a curated selection of primary cell types crucial for constructing a comprehensive picture of TTH biology.

Types	Description
Trigeminal Ganglion (TG) Neurons	The primary sensory neurons that innervate the head and face, central to headache pain transmission.
Dorsal Root Ganglion (DRG) Neurons	Key for studying the general mechanisms of nociception and referred pain.
Cranial Myofibroblasts	Essential for investigating the role of pericranial muscle tension and the release of pro-inflammatory mediators.
Astrocytes & Satellite Glial Cells	Critical for studying neuroinflammation, neuronal support, and the modulation of synaptic activity within the trigeminal system.

Advantages

Choosing Creative Biolabs models translates directly into more robust, efficient, and impactful research.

Generate Clinically Translatable Data

Work with cells that actually participate in the TTH pathway, increasing the likelihood that your findings will translate to human outcomes.

Achieve Unambiguous Results

High-purity models eliminate the noise from cellular contaminants, ensuring your data reflects the biology you intend to study.

Build Reproducible Studies

Our exceptional lot-to-lot consistency means you can trust your results today and confidently build upon them tomorrow.

Save Critical Time and Resources

Skip the time-consuming and uncertain process of primary tissue dissection and cell isolation. Our ready-to-use models let you focus on answering key research questions.

Increase Compound Screening Success

Screen potential analgesics and therapeutics on a more accurate biological system, reducing false positives and improving your hit-to-lead pipeline.

Applications

Our TTH cell models are ideal for a wide range of research applications, empowering discovery across the field.

Applications	Description
Pathophysiology Studies	Investigate the molecular mechanisms of peripheral and central sensitization in TTH.
Drug Discovery & Compound Screening	Perform high-throughput screening for novel analgesics and therapeutic agents targeting pain pathways.
Target Identification & Validation	Confirm the role of specific ion channels, receptors, and signaling molecules (e.g., CGRP, substance P) in nociception.
Myofascial Pain Research	Model the interaction between muscle-derived stress signals and neuronal activation.
Biomarker Discovery	Analyze cellular responses to stimuli to identify potential diagnostic or prognostic biomarkers for TTH.
Neuroinflammation Studies	Explore the role of glial cell activation and inflammatory mediators in the persistence of headache pain.

A picture that presents the nociceptive pathways. (Onan, et al., 2023) (OA Literature)

Fig.1 Pathophysiology of migraine and tension-type headache: nociceptive pathways.¹

FAQs

Can I co-culture different cell types from your portfolio, such as neurons and glial cells?
Yes, in fact, we highly recommend it. Co-culturing our neurons with glial cells (like astrocytes) creates a more physiologically relevant microenvironment. This is crucial for investigating neuro-glial interactions, inflammatory signaling, and modulatory factors that play a key role in TTH pathology. Please contact our technical support for advice on optimized co-culture media and protocols.
Are these primary cells suitable for genetic modification, such as transfection or viral transduction?
Primary neurons are sensitive and can be difficult to transfect with traditional chemical reagents. However, they are highly amenable to gene modification using viral vectors. We recommend using lentiviral or AAV-mediated transduction for efficient gene delivery while maintaining optimal cell health. Optimization of the Multiplicity of Infection (MOI) is advised for best results.
Do you offer bulk purchasing discounts or custom supply solutions for large-scale screening projects?
Absolutely. We understand the demands of high-throughput screening (HTS) and large-scale studies. We offer competitive pricing for bulk orders and can arrange custom, flexible delivery schedules to align with your project timelines. Please contact our sales department to discuss your specific needs and receive a customized quote.
Can I request a custom cell isolation?
We do offer custom services. Please contact our team with your specific requirements, and we can discuss the feasibility of your project.
Can these models be used to study medication-overuse headache?
Yes, they provide an excellent in vitro system to study cellular adaptations and sensitization following chronic exposure to analgesics.

Creative Biolabs empowers the scientific community to solve the most complex challenges in neurology with innovative and reliable cell models. Our Tension Headache platforms provide a powerful, human-relevant system to fast-track the discovery and validation of next-generation therapeutics. Contact our team of expert scientists today to discuss your project needs and allow us to help you select the ideal solution to advance your research.