Primary Rat Neonatal Dorsal Root Ganglion Neurons
Datasheet
Key Features
Cat. No.:
NTV-1225-YJ1
Species
Rat
Cell Type
Neurons
Summary
| Description | Ready-to-use Rat Dorsal Root Ganglion Neurons are high-quality primary sensory neurons prepared for immediate in vitro culture. These cells are an ideal model for neuroscience studies, such as sensory neuron development, function, or neurotoxicity. This product is intended for research use only. |
| Species | Rat |
| Application | Functional Assay |
| Cell Type | Neurons |
| Size | ≥2*10⁵ cells |
| System | Nervous System |
| Growth Conditions |
37°C with 5% CO₂. Adherence Substrate: Culture vessels must be pre-treated with a poly-D-lysine coating. Using laminin as a co-substrate is also recommended. Medium Requirements: Use of a specialized basal medium with a low osmolality (210-240 mOsm/kg H₂O) is mandatory for cell viability. Culture Timeline: Add mitotic inhibitors to the medium precisely 4 hours after plating to prevent glial cell overgrowth. |
| Synonyms | Neonatal Rat DRG Neurons; Rat Neonatal Primary DRG Neurons; P0 Rat DRG Neurons; Rat Sensory Neurons; Primary Rat DRGN; Spinal Ganglion Neurons |
| Biosafety Level | BSL-1 |
| Format | Frozen |
| Shipping | On dry ice or in a liquid nitrogen container. |
| Storage | This product must be stored in liquid nitrogen only. Storage at -20°C or -80°C is prohibited. Avoid repeated freezing and thawing. |
Specifications
| Viability | Do not perform a trypan blue viability count right after thawing. Live cells may also stain, making the count inaccurate. |
| Mycoplasma Testing | Negative |
| Warnings | For your protection, you must wear protective gloves, clothing, and a face mask when handling frozen vials. Bear in mind that some liquid seepage into the vial can occur during storage. Upon thawing, the liquid nitrogen turns into a gas. Due to the nature of nitrogen, this can cause pressure to build up inside the vial, potentially leading to the vial bursting or the cap ejecting. This creates a risk of flying debris. |
| Research Use Only | For Research Use Only. Not For Clinical Use. |
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