Primary Irradiated Mouse Embryonic Fibroblasts
Datasheet
Key Features
Cat. No.:
NTV-1225-YJ12
Species
Mouse
Cell Type
Fibroblasts
Summary
| Description | These are Irradiated Mouse Primary Embryonic Fibroblasts (iMEF) derived from CD1 mouse embryos. They are inactivated by gamma radiation and serve as feeder cells for co-culturing with embryonic and pluripotent stem cells. |
| Species | Mouse |
| Application | Functional Assay |
| Cell Type | Fibroblasts |
| Size | ≥2*10⁶ cells |
| System | Embryonic System |
| Growth Conditions | 37°C with 5% CO₂ |
| Synonyms | iMEFs; Irradiated Primary MEFs; Irradiated Mouse Embryo Fibroblasts; MEF Feeder Layer (Irradiated); iMEF; MEF Feeder Cells; Irradiated MEF |
| Biosafety Level | BSL-1 |
| Format | Frozen |
| Shipping | On dry ice or in a liquid nitrogen container. |
| Storage | This product must be stored in liquid nitrogen only. Storage at -20°C or -80°C is prohibited. Avoid repeated freezing and thawing. |
Specifications
| Mycoplasma Testing | Negative |
| Warnings | For your protection, you must wear protective gloves, clothing, and a face mask when handling frozen vials. Bear in mind that some liquid seepage into the vial can occur during storage. Upon thawing, the liquid nitrogen turns into a gas. Due to the nature of nitrogen, this can cause pressure to build up inside the vial, potentially leading to the vial bursting or the cap ejecting. This creates a risk of flying debris. |
| Research Use Only | For Research Use Only. Not For Clinical Use. |
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