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Calcium Channel Screening Service
Calcium channels, sometimes also known as voltage-gated calcium channels, are membrane potential change-regulated voltage-gated ion channels showing selective permeability to calcium ions. It has a wide tissue distribution and involves signal transduction in cell routine activities. Creative Biolabs presents a remarkable calcium channel screening service to discover the promising compounds of calcium channels for our worldwide clients to facilitate their research.
Introduction of Calcium Channel
Calcium channels, owning the calcium-selective pore in its structure, participate in creating action potential on cell membrane and releasing calcium ions, as the second messenger, to pass the signal for regulating various cell behaviors. In mammals, several voltage-gated calcium channel types are found, like L/N/P/Q/R/T-type calcium channels. Different subunits, including α1, α2δ, β1-4, and γ, compose calcium channels. Among these, the α1 subunit forms the pore structure containing voltage-sensing elements and compound-binding sites for calcium ion transduction and other subunits support the transduction. The α1 subunit is consisted of four homologous domains marked as I to IV and each domain has six transmembrane α-helices labelled as S1 to S6. S4 functions more in gating and sensing voltage than others. Calcium channel defects involves muscles and the nervous system diseases such as malignant hyperthermia and familial hemiplegic migraine, respectively.
Fig.1 The structure of voltage-gated calcium channel and its α1 subunit.1
Calcium Channel Screening Service
Calcium channels exist in the membrane of nearly all excitable cells like muscle and neural cells. Excessive activation of calcium channel leads to an obviously elevated concentration of intracellular calcium ions, followed with high enzyme activity-induced cell structure degradation, called excitotoxicity. Calcium channel screening provides a platform to find the proper modulator for pharmacological research and disease treatment.
Traditional electrophysiological methods like patch clamp have been used in calcium channel screening to reflect the compound-mediated imperceptible electricity change, which provide physiologically significant and highly accurate data on ion channel performance at the single-cell level.
High-throughput screening technology combined non-electrophysiological strategy, like fluorescence-based assay, dramatically improve the throughput of testing candidates from limited compounds to compound library, which perfectly fits the preliminary work of calcium channel research without interested lead compounds in mind and provides a trustworthy and meaningful start point for your interested calcium channel research.
Fig.2 High-throughput screening of calcium channel responsive lead compounds via fluorescence-based assay.
There are different types of calcium channel based on different α1 subunit. Creative Biolabs enables calcium channel screening service based on ready-to-use cell models or our well-established cell line construction platform.
| Channel type | α1 subunit | Main tissue distribution |
| L-type | Cav1.1, Cav1.2, Cav1.3, Cav1.4 | Muscle, bone (osteoblasts), cortical neuron |
| N-type | Cav2.2 | Brain and peripheral nervous system |
| P/Q-type | Cav2.1 | Purkinje neurons, cerebellar granule cells |
| R-type | Cav2.3 | Cerebellar granule cells, neurons |
| T-type | Cav3.1, Cav3.2, Cav3.3 | neurons, bone (osteocytes), thalamus |
Features of Our Service
- Flexible calcium channel cell model
- Tailored assay design and detection
- Trustable screening service with effective cost
- Fast turnaround time and comprehensive data analysis
- Reliable results and best after-sale service
Creative Biolabs, experts in ion channel screening field, can provide custom calcium channel screening service containing both electrophysiological and non-electrophysiological strategies on your interested calcium channels. Feel free to contact us to promote your critical research progress.
In addition to Calcium Channel Screening, Creative Biolabs also provides you with
Reference
- Iftinca, M. C. "Neuronal T–type calcium channels: What's new? Iftinca: T–type channel regulation." Journal of medicine and life 4.2 (2011): 126. Distributed under Open Access license CC BY 2.0, without modification.
For Research Use Only. Not For Clinical Use.