Calcium-activated Channel Screening Service

Calcium-activated channels indicate the ion channels gated by calcium or exhibiting phylogenetical or structural similarity to calcium-gated channels. For the convenience of researchers worldwide, Creative Biolabs, a leader in the field of ion channel screening, provides dependable calcium-activated channel screening services.

Introduction of Calcium-activated Channel

Based on the ion permeability, calcium-activated channels have two well-known types, calcium-activated potassium channels (K_Ca channels) and calcium-activated chloride channels (CaCCs).

• Calcium-activated Potassium Channels

K_Ca channels trigger cell membrane permeability of potassium when intracellular calcium changes and are composed by alpha and beta subtypes. The alpha subunit is a tetramer and contains the structure of the calcium sensing region, pore, and voltage sensor, which is an arginine residue-rich region to sense the charge changes. The beta subunit can be regarded as a channel regulatory subunit. In both healthy and pathological circumstances, K_Ca channels have a role in modulating multiple cellular functions, including oncotransformation.

Fig.1 The structure of calcium-activated potassium channels. Distributed under CC BY-SA 4.0, from Wiki, without modification.

• Calcium-activated Chloride Channels

CaCCs are a heterogeneous group of ligand-gated chloride channels with wide distribution in epithelial and endothelial cell types. CaCCs usually contain 7–10 transmembrane segments and show important roles in fluid secretion, olfactory perception, and neuronal excitability.

Fig.2 The structure of calcium-activated chloride channels. Distributed under CC0 1.0, from Wiki, without modification.

Calcium-activated Channel Screening Service

Calcium-activated channels are implicated in a variety of disease states and are necessary for several physiological processes. Not surprisingly, calcium-activated channels are thought to be interesting targets for treatment. Calcium-activated channel screening is a useful technique for your in vitro pharmacological study of diseases associated to calcium-activated channels. It allows you to find possible activators or inhibitors toward certain calcium-activated channels in which you are interested.

In the realm of ion channel research, electrophysiological techniques such as patch clamp constitute the gold standard. By magnifying the tiny amounts of voltage change brought on by the ions in flow, channel function may be evaluated with accurate resolution in both the temporal and amplitude domains.

When combined with high-throughput screening technology, non-electrophysiological strategies like fluorescence-based assays significantly broaden the pool of potential test subjects, allow for a more efficient and economical method of screening compound libraries, and address the limitations of the labor-intensive and low-throughput electrophysiological method. Nowadays, non-electrophysiological methods are widely used in early research in the field of ion channel screening to identify the activators or inhibitors of certain calcium-activated channels.

Virtual screening can also be considered as an economical way to find theoretically promising ligands because it's assay-free, costly, and high-throughput.

Fig.3 Fluorescence-based assay for high-throughput screening of calcium-activated potassium channel-responsive candidates.

There are several targets for calcium-activated channel screening. Constructing proper cell models and choosing suitable research strategy are keys to identify the ligands with predicted functionalities.

Calcium-activated Potassium Channels

• BK channel with large conductance, representative subtype K_Ca1.1
• IK channel with intermediate conductance, representative subtype K_Ca3.1
• SK channel with small conductance, representative subtype K_Ca2.1

Calcium-activated Chloride Channels

• Accessory family such as CLCA1–4
• Anoctamin family such as ANO1
• Bestrophin family such as BEST1–4

Features of Our Service

• Excellent ability to create cell models
• Tailored assay design and quality-guaranteed assay development
• Reliable and consistent outcomes are produced by timely reporting
• Quick turnaround, economical screening service, and top-notch post-purchase support

Having a great deal of expertise with calcium-activated channel screening research, Creative Biolabs can help you expedite your crucial study by offering various available ion channel screening strategies on the calcium-activated channels that you are interested in. Please contact us if you would like further information.

In addition to Calcium-activated Channel Screening, Creative Biolabs also provides you with the following ligand-gated channel screening services

• CNG Channel Screening
• NMDA Receptor Screening
• nAChR Screening
• GABA_AR Screening
• AMPAR Screening
• P2X Receptor Screening
• TRP Channel Screening
• Sodium-activated Channel Screening

For Research Use Only. Not For Clinical Use.