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Purinoceptor (P2X Receptor) Screening Service
P2X receptors, belonging to the ENaC/P2X superfamily, are ligand-gated ion channels that are open for cation permeation when adenosine 5'-triphosphate (ATP) binding, and have a diverse organism distribution. Experts in the field of ion channel screening, Creative Biolabs provides dependable P2X receptor screening services to researchers globally to promote their P2X receptor studies.
Introduction of P2X Receptor
P2X receptors have a wide tissue distribution and functions in humans, including synaptic transmission in nervous systems and muscle contraction in heart and skeletal muscle. P2X receptors have been divided into seven distinct subunits, each of which has two transmembrane domains, one large extracellular loop, and two intracellular termini. Following its binding to the P2X receptor's extracellular loop, ATP causes a conformational shift in the ion channel's structure, which opens the ion-permeable pore, enabling cations like Na+ and Ca2+ to enter the ion-conducting pore for influx. Numerous clinical disorders affecting the brain, cardiovascular, and urinogenital systems have been linked to abnormal P2X receptor expression.
Fig.1 The structure of P2X receptor. Distributed under Public Domain, from Wiki.
P2X Receptor Screening Service
Given the important roles of P2X receptors in multiple physiological processes, finding modulators of P2X receptors as therapeutic potential targets is promising. Proper cell model construction greatly affects the development of the project. For instance, P2X2 is widely distributed in the autonomic nervous system, while P2X1 receptors are especially noticeable in smooth muscle cells.
You may choose from a variety of approaches for your P2X receptor screening initiatives. Various combinations of methods produce the most reliable findings for your study.
| Methods | In silico strategy | Non-electrophysiological strategy | Electrophysiological strategy | |
| Examples | Virtual screening | Fluorescence-based assay, flux-based assay | Automated patch clamp | Patch clamp |
| Throughput | High | Low to mid | Low | |
| Cost | Low | Mid | High | High |
| Turnaround time | Fast | Mid | Slow | Slower |
| Difficulty | Low | Mid | High | Highly skilled technologist needed |
| Results | Theoretically workable | Less accurate | Physiologically accurate | |
In general, the majority of subunits, named P2X1-7, are capable of forming either homomeric or heteromeric functional receptors. Building a correct and functional cell model is one of the keys to screening the ligands with expected functions.
- Subunit type P2X1 encoded by P2RX1
- Subunit type P2X2 encoded by P2RX2
- Subunit type P2X3 encoded by P2RX3
- Subunit type P2X4 encoded by P2RX4
- Subunit type P2X5 encoded by P2RX5
- Subunit type P2X6 encoded by P2RX6
- Subunit type P2X7 encoded by P2RX7
Features of Our Service
- Both commercially available and specially made P2X receptor cell models are acceptable
- Tailored assay formulation and development with in-time reporting
- Superior, reasonably priced ion channel screening services
- Excellent post-purchase service, trustworthy results, and thorough data analysis
With vast experience in the field of ion channel screening research, Creative Biolabs can accelerate your vital study by offering electrophysiology, non-electrophysiological, or in silico P2X receptor screening services on your target P2X receptor cell models. Feel free to contact us for more details.
In addition to P2X Receptor Screening, Creative Biolabs also provides you with the following ligand-gated channel screening services
- CNG Channel Screening
- NMDA Receptor Screening
- nAChR Screening
- GABAAR Screening
- AMPAR Screening
- TRP Channel Screening
- Calcium-activated Channel Screening
- Sodium-activated Channel Screening
For Research Use Only. Not For Clinical Use.