Cyclic Nucleotide-gated (CNG) Channel Screening Service

Cyclic nucleotide-gated ion channels abbreviated as CNG channels are kind of ion channels reacting to the cyclic nucleotide binding. In both the animal and plant kingdoms, CNG channels are found in multiple cell types. Experts in the field of ion channel screening, Creative Biolabs provides comprehensive CNG channel screening services to scientists worldwide to facilitate their study.

Introduction of CNG Channel

CNG channels, as non-selective cation channels, are widely distributed in the membranes of various cell types and tissues, such as the retina, heart, and olfactory. CNG channels are formed by four subunits surrounding one central pore. Each subunit includes six transmembrane segments marked as S1 to S6, a P-loop, a carboxy terminal region, and an intracellular amino terminal region. The P-loop, together with S6 segment, functions predominantly in ion conduction. The cyclic nucleotide-binding domain, consisting of one β-pleated sheet and two α-helices, is an intracellular domain located at the C-terminus, and is linked to the S6 segment by the C-linker. When cyclic nucleotides are binding plus a hyperpolarization or depolarization event, CNG channels function to finish the ion transduction for cellular activities, for instance hormone release, and sensory transduction, especially in vision and olfaction. Defects in CNG channels lead to various severe diseases like retinitis pigmentosa, achromatopsia, and olfactory impairment.

Fig.1 The structure of CNG channel.Distributed under Public Domain, from Wiki.

CNG Channel Screening Service

The visual and olfactory sensory systems rely heavily on CNG channels for signal transduction in mammals. CNG channel screening allows scientists to identify promising activators or inhibitors of specific CNG channels of interest, and it is an excellent technique for in vitro investigation of CNG channel-related illness occurrence and progress.

In the CNG channel screening services, both electrophysiological methods and non-electrophysiological methods are well-known and commonly used to find the channel modulators. When the project starts from a big compound library or something, non-electrophysiological methods like fluorescence-based assay and ligand binding assay are more recommended because they are high-throughput, time-saving, and cost-saving. When the candidates narrow down to fewer compounds, electrophysiological methods like patch clamps should be preferentially taken into consideration. These are gold standards for ion channel research because of the precise resolution and physiologically related data generation.

Fig.2 Schematic diagram of proposed gating principle of CNG channel from closed to open state.¹

In mammals, CNG channels have six different subtypes. Custom cell overexpression models can be constructed and verified as a guaranteed start point of your CNG channel screening project.

• CNG channel alpha subtype 1
• CNG channel alpha subtype 2
• CNG channel alpha subtype 3
• CNG channel alpha subtype 4
• CNG channel beta subtype 1
• CNG channel beta subtype 3

Features of Our Service

• Outstanding cell model construction capacity
• Custom assay design and quality-guaranteed assay development
• In-time reporting generating trustworthy and repetitive results
• Rapid turnaround time, cost-efficient screening, and best after-sale service

To accelerate your crucial research progress, Creative Biolabs, which has vast experience in ion channel screening research, can offer both electrophysiological and non-electrophysiological screening strategies on your interested CNG channels. Do not hesitate to contact us for more details.

In addition to CNG Channel Screening, Creative Biolabs also provides you with the following ligand-gated channel screening services

• NMDA Receptor Screening
• nAChR Screening
• GABA_AR Screening
• AMPAR Screening
• P2X Receptor Screening
• TRP Channel Screening
• Calcium-activated Channel Screening
• Sodium-activated Channel Screening

Reference

1. Mazzolini, Monica, et al. "The gating mechanism in cyclic nucleotide-gated ion channels." Scientific Reports 8.1 (2018): 45. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only. Not For Clinical Use.